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DBCO-PEG10000-Silane 二苯并环辛炔-聚乙二醇-硅烷 含有硅烷和二苯并环辛炔的PEG
发布时间:2023-06-19     作者:zhn   分享到:

含有硅烷和二苯并环辛炔的PEG DBCO-PEG10000-Silane

产品设备简称:DBCO-PEG-Silane汉语明称:二苯并环辛炔-聚乙二醇-硅烷PEG大原子核量:1k,2k,3.4k,5k,10k同一大原子核量可自定义储存方式状况:≤-4℃温度干涩存有產品外观设计: 膏状或粘稠介质,考量于氧分子量较为常用容剂:可溶性高,溶于水的于大局部充分容剂,如:DCM、DMF、DMSO、THF孩他。在水有良好 的可溶性高,溶于水的性DBCO广泛用于无铜点击量化学生理现象。在不会铜促使剂的现象下,与叠氮有机物的生理现象相当迅猛。种生理现象是相当迅猛,性情温和,和生物体混溶性。银离子液体具体条件下,DBCO不错迅速的的酰化氨基官能团;对八种构成20种绿色酪氨酸的多肽底物调查找到,DBCO酰化不良想法迟钝高选用性地发生的在Lys的氨基上,而不会作用同一酪氨酸。时候,银离子液体DBCO酰化不良想法迟钝不错**地掩盖糖肽、BSA蛋白质酶、糖蛋白质酶RNase B 和IgG表面抗原等,体现出最好的底物使用性。除此以外,银离子液体酰化不良想法迟钝不错变现Ph.D.12商业地产噬菌体库的掩盖且不会作用噬菌体浸染魅力,阐明该具体方法存在软件于活生态学体系中中的升值空间。在响应缓冲器液中配制含叠氮化物的图纸。将DBCO共轭物加到含叠氮化物的图纸中。也可以:在丰度生化化学试剂的1.5-3.0摩尔当量中加1摩尔当量的上限生化化学试剂。将响应在空调温度下于4华氏度温育2-4几一小时或2-12几一小时。現在也可以将响应纯化。DBCO is used for copper free click chemistry. Without copper catalyst, the reaction with azide compound is very rapid. This reaction is very rapid, mild, and biocompatible.Under silver catalysis, DBCO can quickly acylate amino groups; The study of three peptide substrates containing 20 natural amino acids found that the DBCO acylation reaction occurred on the amino acid of Lys with high selectivity, without affecting other amino acids. At the same time, silver catalyzed DBCO acylation reaction can efficiently modify glycopeptides, BSA proteins, glycoprotein RNase B and IgG antibodies, showing good substrate applicability. In addition, silver catalyzed acylation can achieve the modification of commercial phage library of Ph.D.12 without affecting the infection activity of phages, indicating that this method has potential application in living biological systems.The sample containing azide was prepared in the reaction buffer. DBCO conjugate was added to the sample containing azide. It is recommended to add a limiting reagent of 1 mole equivalent to the 1.5-3.0 mole equivalent of the abundance reagent. The reaction was incubated at 4 ℃ at room temperature for 2-4 hours or 2-12 hours. The reaction can now be purified.DBCO-GPC二苯基环辛炔-甘磷酸胆碱DBCO-PFP二苯基环辛炔-五氟苯酚DBCO-Benzaldehyde二苯基环辛炔-苯室内甲醛DBCO-Mesylate 二苯基环辛炔-甲磺酸酯DBCO-TPP二苯基环辛炔-磷酸三苯酯DBCO-Phenyl二苯基环辛炔-苯基DBCO-acid二苯基环辛炔-磷酸DBCO-GA二苯基环辛炔淡化甘草次酸DBCO-Cyclodextrin二苯基环辛炔偶联环糊精DBCO-Dopamine 二苯基环辛炔装饰多巴胺DBCO-Ferrocene二苯基环辛炔体现二茂铁应用领域:科研项目纯净度:≥95%永久保存时光: 2年主产地:上海产生商:郑州pg电子娱乐游戏app 创科生物工程社会是有限的大公司考虑重大事项:仅使用在科学研究不使用在人体内