文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

A poly(lactic-co-glycolic acid)-poly(ethylene glycol)-carboxyl (PLGA-PEG-COOH) and gelatin-based microparticles (MPs) dual delivery system for release of bone morphogenetic protein-2 (BMP-2) and insulin-like growth factor-1 (IGF-1) is developed. The delivery system is characterized based on its morphology, loading capacity, encapsulation efficiency, and release kinetics. The effects of electron beam (EB) sterilization on BMP-2 and IGF-1-loaded MPs and their biological effects are examined. The synergistic effect of a controlled dual release of BMP-2 and IGF-1 on osteogenesis of human mesenchymal stem cells (MSCs) is evaluated. Encapsulation efficiency of growth factors into gelatin and PLGA-PEG-COOH MPs is in the range of 64.78% to 76.11%. E-beam sterilized growth factor delivery systems are effective in significantly promoting osteogenesis of MSCs, although E-beam sterilization decreases the bioactivity of growth factors in MPs by ≈22%. BMP-2 release behavior from gelatin MPs/PEG hydrogel shows a faster release (52.7%) than that of IGF-1 from the PLGA-PEG-COOH MPs/PEG hydrogel (27.3%). The results demonstrate that the gelatin and PLGA-PEG-COOH MPs-based delivery system can realize temporal release of therapeutic biomolecules by incorporating different growth factors into distinct microparticles. EB sterilization is an accessible method for sterilizing growth factors-loaded carriers, which can pave the way for implementing growth factor delivery in clinical applications.

搭建打了个种使用放出骨价值形式发生了蛋白酶-2（BMP-2）和胰岛素样滋生指数公式-1（IGF-1）的聚乳酸-聚乙二醇-羧基（PLGA-PEG-COOH）和明胶基水分子（MP）双递送整体。递送整体的特殊性就是：其价值形式、电机负载功能、包封效应和放出动力系统学。科学研究了电子器件束（EB）灭菌处理对环境下BMP-2和IGF-1的MP的反应和微分子生物学效果。评价指标了BMP-2和IGF-1的受控多重宣泄对人世充质干神经元（MSCs）成骨的推进帮助。

生长因子在明胶和PLGA-PEG-COOH MP中的包封效率在64.78%至76.11%之间。电子束灭菌的生长因子递送系统能有效显著促进MSCs的成骨，尽管电子束灭菌会使MP中生长因子的生物活性降低约22%。明胶MPs/PEG水凝胶的BMP-2释放行为比PLGA-PEG-COOH MPs/PEG凝胶的IGF-1释放行为（27.3%）更快（52.7%）。

結果表达，确认明胶和PLGA-PEG-COOH MP的递送体统不错确认将其他的萌发因素掺杂其他的水分子结构当中保持改善性菌物分子结构的还释放出来。EB消毒都是种能作于对存储萌发因素的质粒载体开始消毒的方法步骤，不错为在临床研究应用领域中快速执行萌发因素递送刮平路段。相应推存：N3-PEG-SVAFA-PEG-N3N3-PEG-SSN3-PEG-SGPhosphoric acid-PEG-N3N3-PEG-PAA(1.8K)-DAN3-PEG-NHSN3-PEG-AlkeneACA-PEG-N3AC-PEG-N3AlKyne-PEG-N3COOH-PEG-N3MA-PEG-N3N3-PEG-EPON3-PEG-OH之内文章标题內容收入以及中文核心期刊或论文参考文献，请谅解知识产权侵权请连续各位移除！