专著：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy创作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie文献综述联接： 引言：A novel folate (FA) receptor-targeted superparamagnetic Fe3O4 nanoparticles (SPIONs) codelivering cisplatin (CDDP) and tissue factor pathway inhibitor-2 (TFPI-2) plasmid DNA (pDNA) was constructed. The core shell nanocomposites (FA-PEG-PEI@SPION-CDDP-TFPI-2) were composed of superparamagnetic Fe3O4 core that binds CDDP and TFPI-2 shell that combines with folate-polyethylene glycol-polyethyleneimine (FA-PEG-PEI) via electrostatic interaction. The shell containing FA-PEG-PEI and TFPI-2 plasmid was synthesized through amidation reaction and electrostatic adsorption and the core containing SPION-CDDP was modified by aldehyde sodium alginate. Proton nuclear magnetic resonance and Fourier transform infrared spectra on FA-PEG-PEI polymers showed characteristic peaks of various metabolites in corresponding frequency. Transmission electron microscopy image of FA-PEG-PEI@SPION-CDDP-TFPI-2 nanoparticles demonstrated a near-monodisperse spherical morphology, while dynamic light scattering studies indicated an intensity-average diameter of 149.5 nm. Zeta potential was 14.89 ± 1.83 mv and the final concentration of loaded CDDP was 100 ug/ml. Gel electrophoresis data showed that the nanocomposite would protect TFPI-2 pDNA from being digested by DNases. Compared with CNE-2 cells, the good targetability and better gene transfection efficiency (57.9%) were detected by Prussian blue iron stain and fluorescence analysis in HNE-1 cells in vitro. The results suggested the potential application of FA-PEG-PEI@SPION-CDDP-TFPI-2 as a multifunctional anticancer nanomedicine on targeting therapy for FR positive NPC.

融合了了种创新孕妇叶酸（FA）多巴胺受体靶向药物的超顺磁铁Fe3O4納米水粒子（SPION），其可同时递送顺铂（CDDP）和组织性系数渠道克注射剂-2（TFPI-2）质粒DNA（pDNA）。核壳纳米级分手后复合装修材料(FA-PEG-PEI@SPION-CDDP-TFPI-2)由整合CDDP的超顺剩磁Fe3O4核和依据静电放电能之间效果与备孕叶酸聚乙二醇聚丁二烯亚胺（FA-PEG-PEI）整合的TFPI-2壳组成的。依据酰胺化反應和静电放电能活性炭吸附自动合成了包有效FA-PEG-PEI和TFPI-2质粒的设备壳，一起用水藻酸钠醛表达了包有效SPION-CDDP的重要。FA-PEG-PEI整合物的质子核磁震动和傅里叶改换红外光谱仪屏幕上显示了相关工作频率下各种各样的代谢率物的特性峰。散射智能高倍显微镜画像FA-PEG-PEI@SPION-CDDP-TFPI-2nm再生颗粒展示出近于单分布的圆形形状，而动态图片光散射理论研究反映出其效果均匀截面积为149.5 nm.Zeta电位差为14.89±1.83 mv，过载CDDP的结果英文盐浓度为100 疑胶电泳数值凸显，nm分手后复合文件还可以确保TFPI-2 pDNA不会受到DNA酶的吸收。与CNE-2肿瘤血细胞对比，普鲁士蓝铁印染和荧光研究在HNE-1肿瘤血细胞中查重到优良的靶向药物性和更加好的染色体转染生产率（57.9%）。重要性推见：PLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCMALKENE-PEG-OH CAS No.: 27274-31-3OH-PEG-PI(38K)PLGA(5K)-PEG-OHOH-PEG-AA-C30FA-PEG-OH超过的文章东西起源各项杂志期刊或文献资料，比如侵犯商标权请关系我们大家删去！