文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

Proper particle diameter [25, 26] and positive surface charge of nanodrug play an important role in cellular uptake of drug and enhancement target-oriented effects [17]. The particle size of FA-PEG-PEI@SPION-CDDP-TFPI-2 complexes was determined using TEM (Figure 4(a)). TEM micrograph illustrated monodisperse spherical particles with an average diameter of 55.2 ± 8.4 nm, the sizes of which contribute to avoiding phagocytosis by macrophages, renal excretion, and aggregation in the bone marrow. There were several SPION particles at an average diameter of 8 nm arranged as a ring inside the complex. Negligible sedimentation was observed in the complex liquid with one-year observation, which suggested excellent stability of the system. The TEM EDS in Figure 4(b) verified the presence of Pt and Fe elements in FA-PEG-PEI@SPION-CDDP-TFPI-2 complexes, suggesting a potent combination between CDDP and SPION. The Fe content and CDDP loading amount were 5.35 mg/ml and 100 ug/ml, respectively, as counted according to corresponding standard curves by colorimetry.

纳米级药剂剂量的应当比界面积和正界面自由电荷在血细胞摄入药剂剂量和增进靶点滞后效应中起侧重于要功效。比界面积FA-PEG-PEI@SPION-CDDP-TFPI-2使用的TEM核查混合物。TEM显微照片图片展现了一般的的的直径为55.2±8.4纳米的单扩散圆球形小粒 nm，其大大小小益于避开巨噬组织的夺取用处、肾脏排泄物和骨髓中的聚积。有几个比较一般的的的直径为8纳米的SPION小粒 nm在和好物内排顺成环。经一年时间的看，在非常复杂的液状体中看到可改变的奠定，这表述该机系统有优秀的稳定义性。TEM EDS安全验证了Pt和Fe原子在FA-PEG-PEI@SPION-CDDP-TFPI-2组合物，认为CDDP和SPION直接来源于效果的组合。铁成分和CDDP给出量为5.35 mg/ml和100 ug/ml，给出合适的标准单位的身材曲线经过比色法计算。想关建议：mPEG-PLGA(1K)mPEG-PLGA(10K)mPEG-PLGA(20K)mPEG-PEI(1K)mPEG-PCL(1K)mPEG-PLGA(2K)mPEG-PLGA(4K)mPEG-PiperazinemPEG-PLA(10K)mPEG-PLA(5K)mPEG-C18H37mPEG-GlutathionemPEG-HistidinemPEG-PSBmPEG-PLGA(5K)mPEG-DTPA上优秀文章资源来源地各种类型杂志期刊或论文，告之抄袭请关系咱们刪除！