文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

The FA-PEG-PEI@SPION-CDDP was constructed with SPION-CDDP (core) and FA-PEG-PEI (shell). Numerous detached electropositive amino groups in the shell covered SPION-CDDP nanoparticles were able to electrostatically adsorb electronegative TFPI-2 pDNA. To explore the binding ability of FA-PEG-PEI@SPION-CDDP with TFPI-2 pDNA, gel electrophoresis was observed in Figure 3(a). It was found that with the increasing mass ratio of FA-PEG-PEI@SPION-CDDP/TFPI-2 {Figure 3(a)~B (1 : 4) → C (1 : 2) → D (1 : 1)}, more TFPI-2 was blocked in sample well. When the mass ratio was equal to or more than 2 (Figure 3(a)~E), TFPI-2 pDNA was restricted completely in the well, indicating entirely adsorption and encapsulation by FA-PEG-PEI@SPION-CDDP. As a result, the relative mass ratio was determined as 2 : 1 to synthesize the final complex. To investigate the protection of TFPI-2-loaded complex (FA-PEG-PEI@SPION-CDDP-TFPI-2) against DNase-I degrading, FA-PEG-PEI@SPION-CDDP was mixed with overdose TFPI-2, then various content of DNase-I was added to run electrophoresis. As shown in Figure 3(b), compared with TFPI-2 migration without DNase-I in Figure 3(a)~B, the more DNase-I was added, the more detached TFPI-2 was degraded with more fade band of TFPI-2 migration. When DNase-I was equal to or higher than 15 units, the detached TFPI-2 (Figure 3(b)~J, K) was entirely digested except for the sample well. This evidence shed light on the enzymatic hydrolysis of DNase-I in TFPI-2 pDNA and indicated that FA-PEG-PEI@SPION-CDDP is feasible in adsorbing TFPI-2 and protecting pDNA from degradation. Thus pDNA could avoid digestion by DNase in media or blood before reaching the targeted cells, leading to an efficient transfection in tumors.

这些FA-PEG-PEI@SPION-CDDP用SPION-CDDP（核心内容）和FA-PEG-PEI（外层）倡导。壳覆盖住的SPION-CDDP微米顆粒中的有很多分离出来的正电氨基就可以静电感应粘附电负性TFPI-2 pDNA。生命的进化其构建水平FA-PEG-PEI@SPION-CDDP而言TFPI-2 pDNA，凝露电泳。看见由于质理比的曾加FA-PEG-PEI@SPION-CDDP/TFPI-2{图3（a）~B（1:4）→C（1:2）→D（1:1）}，更好的TFPI-2在土样孔中被恰恰能阻隔。当质理比=或高于2时，TFPI-2 pDNA已经限定在孔中，表示已经被离心分离和包封FA-PEG-PEI@SPION-CDDP.结果显示，相较质理比被确定好为2 : 1 提炼既定的结合物。TFPI-2负载电阻复合型物的护理能力分析(FA-PEG-PEI@SPION-CDDP-TFPI-2)抗DNase-I分解，FA-PEG-PEI@SPION-CDDP与过度TFPI-2比调，第三进入各个占比的DNase-I来电泳。中找不到DNase-I的TFPI-2搬迁优于，添加图片的DNase-I越久，TFPI-2的挥发能力越高，TFPI-1搬迁的衰减带越久。当DNase-I值为或要高于18个的单位时，除供试品孔外，拆分的TFPI-2仍然被转化。一项视听资料折射出了TFPI-2 pDNA中DNase-I的酶电离，并认为FA-PEG-PEI@SPION-CDDP在吸附物TFPI-2和庇护pDNA不会受到化学降解方向是准许的。如此，pDNA可以以免 在来到靶体细胞之后被培养教育基或血液循环系统中的DNase消化不好，为了在癌肿中改变高转染。涉及举荐：OH-PEG-SSOH-PEG-SGLA-PEG-OHN-(3-hydroxypropyl) phthalimide-PEG-OHBenzyl-PEG-OHPLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCM以内篇文章的内容来原各大学术期刊或论文资料，如不侵犯肖像权请保持联系小编误删！