文献：Preparation and Characterization of Folate-Targeted Fe3O4 Nanoparticle Codelivering Cisplatin and TFPI-2 Plasmid DNA for Nasopharyngeal Carcinoma Therapy

作者：Juan Zhang, Huanhuan Weng, Xiangwan Miao, Quanming Li, Siqi Wang, Huifen Xie, Tao Liu, Minqiang Xie

文献链接：//onlinelibrary.wiley.com/doi/full/10.1155/2017/2849801

摘要：

The FA-PEG-PEI@SPION-CDDP was constructed with SPION-CDDP (core) and FA-PEG-PEI (shell). Numerous detached electropositive amino groups in the shell covered SPION-CDDP nanoparticles were able to electrostatically adsorb electronegative TFPI-2 pDNA. To explore the binding ability of FA-PEG-PEI@SPION-CDDP with TFPI-2 pDNA, gel electrophoresis was observed in Figure 3(a). It was found that with the increasing mass ratio of FA-PEG-PEI@SPION-CDDP/TFPI-2 {Figure 3(a)~B (1 : 4) → C (1 : 2) → D (1 : 1)}, more TFPI-2 was blocked in sample well. When the mass ratio was equal to or more than 2 (Figure 3(a)~E), TFPI-2 pDNA was restricted completely in the well, indicating entirely adsorption and encapsulation by FA-PEG-PEI@SPION-CDDP. As a result, the relative mass ratio was determined as 2 : 1 to synthesize the final complex. To investigate the protection of TFPI-2-loaded complex (FA-PEG-PEI@SPION-CDDP-TFPI-2) against DNase-I degrading, FA-PEG-PEI@SPION-CDDP was mixed with overdose TFPI-2, then various content of DNase-I was added to run electrophoresis. As shown in Figure 3(b), compared with TFPI-2 migration without DNase-I in Figure 3(a)~B, the more DNase-I was added, the more detached TFPI-2 was degraded with more fade band of TFPI-2 migration. When DNase-I was equal to or higher than 15 units, the detached TFPI-2 (Figure 3(b)~J, K) was entirely digested except for the sample well. This evidence shed light on the enzymatic hydrolysis of DNase-I in TFPI-2 pDNA and indicated that FA-PEG-PEI@SPION-CDDP is feasible in adsorbing TFPI-2 and protecting pDNA from degradation. Thus pDNA could avoid digestion by DNase in media or blood before reaching the targeted cells, leading to an efficient transfection in tumors.

这位FA-PEG-PEI@SPION-CDDP用SPION-CDDP（关键）和FA-PEG-PEI（塑料壳）建立。壳盖住的SPION-CDDPnm颗粒剂中的诸多区分的正电氨基要能靜電吸收电负性TFPI-2 pDNA。挑战其切合意识FA-PEG-PEI@SPION-CDDP相对TFPI-2 pDNA，妇科凝胶电泳。得知随之高产品的品质比的提升FA-PEG-PEI@SPION-CDDP/TFPI-2{图3（a）~B（1:4）→C（1:2）→D（1:1）}，一些的TFPI-2在样机孔中被传导。当高产品的品质比相等于或大于等于2时，TFPI-2 pDNA全局限在孔中，证明全被吸附物和包封FA-PEG-PEI@SPION-CDDP.最后，较为高产品的品质比被判定为2 : 1 聚合后面的复合型物。TFPI-2过载结合物的保护性目的探究(FA-PEG-PEI@SPION-CDDP-TFPI-2)抗DNase-I挥发，FA-PEG-PEI@SPION-CDDP与超量TFPI-2结合，再添加各个含锌量的DNase-I展开电泳。中不存在DNase-I的TFPI-2迁徙相对来说，含有的DNase-I就越，TFPI-2的挥发水平越高，TFPI-1迁徙的衰减带就越。当DNase-I等于6或高过1几个计量单位时，除印刷品孔外，分开的TFPI-2全部被化解。上述离婚证据阐释了TFPI-2 pDNA中DNase-I的酶水解反应，并表述FA-PEG-PEI@SPION-CDDP在吸TFPI-2和保护性pDNA不被受到溶解的方面是必须的。为此，pDNA可以防范在运到靶神经元已经被激发基或血细胞中的DNase消化不好，于是在肿癌中建立效率高转染。重要性最新推荐：OH-PEG-SSOH-PEG-SGLA-PEG-OHN-(3-hydroxypropyl) phthalimide-PEG-OHBenzyl-PEG-OHPLGA(20K)-PEG-OHPCL(5K)-PEG-OHPLA(2K)-PEG-OHOH-PEG-PLA(3K)6-NO2DA-PEG-OHOH-PEG-AAOH-PEG-SCM以上知识散文知识由来四种杂志或医学文献，如果版权侵权请结合大家清空！