资料:Gutenberg-style printing of self-assembled metal nanoparticle Aarays: electrostatic nanoparticle immobilisation and DNA-mediated transfer小说作品： Zheng, Yuanhui文献综述外部链接：提要： HS–PEG–COOH was diluted in citrate solution while stirring at room temperature. An amount of DTT was added in the solution with molar ratio DTT/HS–PEG–COOH about 1/1. Here the DTT produces the free sulfhydryl bonds. The solution DTT–HS–PEG–COOH then was added into primary AuNPs solution while stirring at room temperature for about 15 min. The minimum amount of HS–PEG–COOH required to stabilize AuNPs was determined by the method similar to the BSA case. Two serial dilutions of the HS–PEG–COOH–AuNPs (Au@PEG) at pH=4 and pH=6.5 were prepared, varying the quantities of HS–PEG–COOH from 0 to 150 μg in 1 ml of AuNPs solution (μg  ml −1), and followed by UV-VIS spectrophotometric analysis. The minimum amount of HS–PEG–COOH necessary to coat AuNPs was deduced graphically from the concentration with which the absorbance at pH=4 becomes nearly constant. All of these solutions were measured by UV-Vis spectrophotometry. The size and shape of AuNPs were characterized by a transmission electron microscope (TEM).

在常温下绞拌机的同時，将HS-PEG-COOH直接稀释就可以在柠檬百香果酸盐悬浊液中。在悬浊液里添进入一些 量的DTT，DTT/HS–PEG–COOH的摩尔比约为1/1。在这些里，DTT诞生氧化钙含量巯基键。第二步将DTT-HS-PEG-COOH悬浊液进入到初中级AuNP悬浊液中，同時在常温下绞拌机约14分 min。安全AuNP需用的HS-PEG-COOH的最低量是利用如此于BSA的技巧断定的。HS-PEG-COOH-AuNPs的每种编稀释饱和稀硫酸液(Au@PEG)在pH=4和pH=6.5的经济条件下，将HS-PEG-COOH的量从0改变到150 μg/1 ml金奈米粒子饱和稀硫酸（μg  ml-1），最后借助UV-VIS分光光度研究分析。依据pH=4时吸光度可以说节流过程的溶度，以外观措施推证出涂覆AuNP所要的最短HS-PEG-COOH量。所以这样的饱和稀硫酸都借助分光光度计-看得见分光光度法借助了检测。借助电子技术散射电子技术显微镜观察（TEM）对金奈米粒子的尺码和外观借助了分析方法。相应推介：CLS-PEG-SilaneCLS-PEG-FITCCLS-PEG-BiotinCLS-PEG-MalCLS-PEG-FACLS-PEG-SHCLS-PEG-NH2CLS-PEG-COOHCLS-PEG-SCmPEG-CLS8-ArmPEG-CLS4-ARMPEG-CLSCLS-PEG-CLS上面的论文主要内容由来各种各样中文核心期刊或文章，假如侵犯商标权请练习人们删除文件！