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SH-PEG-COOH介导的金纳米棒多功能表面修饰及细胞穿透肽偶联
发布时间:2025-07-23     作者:zyl   分享到:
论文:Surface Functionalized Gold Nanorods: Tracking and Observing Live Cell via Three Optical Signals作著:Qiu, Xiaozhong; Wang, Yaling; Cui, Yanyan; Sun, Zhipeng; Liu, Ru; Huang, Wenhua; Zhao, Feng; Zu, Yan; Yuan, Hui; Chang, Xueling; Gao, and Xueyun学术论文连接://www.ingentaconnect.com/contentone/asp/jnn/2012/00000012/00000009/art00004小结:Surface functionalized gold nanorods (GNRs) with three optical signals and the ability to track and observe live cells was investigated. The Cetyltrimethyl Ammonium Bromide (CTAB) was used as the shape-directing agent to develop GNRs with uniform size and aspect ratio. The as-synthesized GNRs can strongly enhance the Raman signal of the attached MGITC on the GNRs. Polyethylene glycol (SH-PEG-COOH) and SH-PEG-FITC were attached with GNRs through ligand exchange with CTAB. They can stabilize the GNRs, reduce the cytotoxicity and provide fluorescence signal. A cell-penetrating peptide was covalently conjugated with SH-PEG-COOH to assist the GNRs to enter living cell. After cultured with the MCF-7 cells, the surfaced functionalized Au nanorods can enter the MCF-7 cells and be tracking with enhanced MGITC Raman signal, FITC fluorescence, and Rayleigh scatter signal.

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分析了更具这三种光数据走势的面上功能性化金奈米棒(GNR)或者跟进和洞察分析活上皮细胞的效率。选用第十六烷基三甲医院基溴化铵(CTAB)用作形状图片指引剂,搭建出尺码和纵橫比均的GNR。结合的GNR还可以微弱资料GNR上支承的MGITC的拉曼数据走势。聚乙二醇(SH-PEG-COOH)和SH-PEG-FITC完成与CTAB的配体交互与GNR联系。他们就能够安全GNR,减小肿瘤组织毒素并给予荧光4g卫星信号灯。肿瘤组织穿透力肽与SH-PEG-COOH共价综合,以助力GNR开始活肿瘤组织。与MCF-7肿瘤组织分着养成后,表面上官能化的Au納米棒就能够开始MCF-7肿瘤组织,互用增強的MGITC拉曼4g卫星信号灯、FITC荧光和瑞利散射4g卫星信号灯做好监测。涉及到的推薦:Palmitic acid-PEG-MalPalmitic acid-PEG-NH2Palmitic acid-PEG-SCRB-PEG-DSPESTA-PEG-CHOSTA-PEG-COOHSTA-PEG-FASTA-PEG-FITCSTA-PEG-MalSTA-PEG-NBDSTA-PEG-NH2上短文相关内容来源于当下期刊论文或学术论文,深表歉意侵犯著作权请沟通我们大家删出!