文章：MICRORHEOLOGICAL QUANTIFICATION OF CELL-MEDIATED REMODELING OF POLYMER-PEPTIDE HYDROGELS IN RESPONSE TO CHEMICAL CUES文章环节： 小结：The next chapter describes the development of a new technique to create hydrogels with tethered concentration gradients of polymers or proteins. This technique also enables us to measure the diffusion coefficient of solutes in hydrogels. Hydrogels with tethered concentration gradients of cell signaling molecules can be used to direct cell migration from the scaffold to the wound after implantation. A rectangular prism is the basis of our microfluidic device. Before using the microfluidic device to create hydrogels with a tethered concentration gradient of TNF-α, we make concentration gradients with a model polymer, Fluorescein poly(ethylene glycol)-thiol (FITC-PEG-SH), to validate the technique. We first make a calibration curve by measuring hydrogels with known concentrations of FITC-PEG-SH and measure their arbitrary brightness using a laser scanner. This calibration curve relates brightness with tethered molecule concentration. We form hydrogels with tethered concentration gradients of FITC-PEG-SH by allowing the molecule to diffuse for 6, 24 and 48 hours. Hydrogels with tethered concentration gradients of FITC-PEG-SH are placed on the laser scanner and spatial brightness is measured. The calibration curve is then used to back-calculate local concentrations every 25 micrometers along the hydrogel. Next, we curve-fit a solution of Fick’s second law of diffusion to calculate the effective diffusion coefficient of FITC-PEG-SH. In the FITC-PEG-SH experiments, we hypothesize that steady state diffusion is reached when a gradient forms for 24 hours. We use the same method to make concentration gradients of thiolated and dyed TNF-α tethered into hydrogels. 

该工艺行手工制造能力兼具高聚物物或核脂肪酸桎梏溶度指数公式的水妇科抑菌凝露。该工艺还使小编会測量水妇科抑菌凝露中溶质的扩散作用指数公式。兼具癌细胞膜表现分子式系留溶度指数公式的水妇科抑菌凝露都可以于在植入广告后优化癌细胞膜从支吊架移动到伤口发炎。方形反射镜是小编微水射流动力配置的基础性。在选用微水射流动力配置手工制造能力兼具TNF-α桎梏溶度指数公式的水妇科抑菌凝露以往，小编用整治高聚物物荧光素聚乙二醇硫醇（FITC-PEG-SH）打造溶度指数公式，以验正该工艺。.我1施用测试相等渗透压的FITC-PEG-SH水妇科疑胶来制作去校正弧线，并施用脉冲光检测仪测试其不同色彩饱和度。该去校正弧线将色彩饱和度与系留原子渗透压沟通上去。.我施用让原子分散作用6、24和48h，去存在FITC-PEG-SH束搏渗透压等度的水妇科疑胶。将存在FITC-PEG-SH束搏渗透压等度的水妇科疑胶安装在脉冲光检测仪上，并测试区域空间色彩饱和度。其次施用去校正弧线沿水妇科疑胶每252um反相换算方式连续部分区域渗透压。收起来，.我对菲克第二步分散作用定理的解去弧线线性拟合，以换算方式FITC-PEG-SH的效果分散作用公式。在FITC-PEG-SS实验报告中，.我统计假设当等度去24h时实现稳定分散作用。.我施用同样的形式使巯基化和脱色的TNF-α的渗透压等度稳定在水妇科疑胶中。衡量比分析预测实际值快一种数据级的更好发展数值。在新建众多水凝胶的作用的作用的作用的作用复制出品后，你们假设检验最快的速度速的发展是因此TNF-α和水凝胶的作用的作用的作用的作用交连剂相互间的防静电诱惑。这个最简单的方法应该扩大或缩减，以提纯享有系留氧化还原电位梯度方向的水凝胶的作用的作用的作用的作用，用以人体细胞递送，或衡量水凝胶的作用的作用的作用的作用中汇聚物或球胆固醇的发展数值。相应的比较适合：AA-PEG-AAAlkyne-PEG-SVAPLGA(2K)-PEG-SCDBCO-PEG-SVAGala-PEG-SCMGluc-PEG-SCMSCM-S-PEG-S-SCMSVA-PEG-SVASPA-PEG-SPASAS-PEG-SASGAS-PEG-GASSG-PEG-SG之上本文网站内容种类各种中文核心期刊或文献综述，予以图片侵权请认识人们移除！