论文：Polymeric Micelles Modified by Folate-PEG-Lipid for Targeted Drug Delivery to Cancer Cells In Vitro小编： Hayama, Akihiro; Yamamoto, Tatsuhiro; Yokoyama, Masayuki; Kawano, Kumi; Hattori, Yoshiyuki; Maitani, Yoshie论文参考文献联结：//www.ingentaconnect.com/contentone/asp/jnn/2008/00000008/00000006/art00040引言：A novel technique was developed for the formation of ligand-targeted polymeric micelles that can be applicable to various ligands. For tumor-specific drug delivery, camptothecin (CPT)-loaded polymeric micelles were modified by folate to produce a folate-receptor-targeted drug carrier. Folate-linked PEG5000-distearoylphosphatidylethanolamine (folate-PEG5000-DSPE) was added when preparations of drug-loaded polymeric micelles, resulting in folate ligands exposed to the surface. Folate-modified CPT-loaded polymeric micelles (F-micelle) were evaluated by measuring cellular uptake using a flow cytometer, fluorescence microscopy, and confocal laser scanning microscopy, and by cytotoxicity measurement. The results revealed that F-micelle showed higher cellular uptake in KB cells over-expressing folate receptor (FR) and higher cytotoxicity compared with non-folate modified CPT-loaded polymeric micelles (plain micelles) in KB cells, but not in FR-negative HepG2 cells. This result indicated that polymeric micelles were successfully modified by the folate-linked lipid.

開發一种新技術，主要适用确立可适主要适用各种类型配体的配体靶向抗癫痫药物疗法汇聚物胶束。对于那些癌症特女性朋友中成药递送，载荷喜树碱（CPT）的汇聚物胶束被DHA装饰，产生了DHA肾上腺素受体靶向抗癫痫药物疗法中成药形式。当提纯根据用药的缔合物胶束时，成为孕妇孕妇叶酸片相连接的PEG5000二硬脂酰磷脂酰工业乙醇胺（Folate-PEG5000-DSPE），使得孕妇孕妇叶酸片配体展现在表明。依据选用普鲁士蓝染色神经元仪、荧光体视显微镜和共聚交二氧化碳激光扫一扫体视显微镜校正神经元摄取量或神经元毒素校正来风险评估孕妇孕妇叶酸片体现的CPT根据缔合物胶束（F-胶束）。然而表面，与KB神经元中予以备孕备孕叶酸呈现的CPT载荷汇聚物胶束（常见的胶束）比起来，F-胶束在过表明备孕备孕叶酸肾上腺素受体（FR）的KB神经元中行为 出更加高的神经元摄入率和更加高的致癌性，但在FR弱阳的HepG2神经元中则也不。此种的结果体现了，备孕叶酸衔接的脂质成就地表达了缔合物胶束。相应建议：DSPE-PEG-AlkyneDSPE-PEG-RhodamineDSPE-RhodamineDSPE-PEG-SHDSPE-PEG-SPDPDSPE-SPDPDSPE-PEG-TFPDSPE-PEG13-TFPDSPE-PEG-Vinylsulfone这好的文章介绍特征各个中文核心期刊或论文，请谅解知识产权侵权请链接我册除！