医学文献：Extracellular Vesicle Crosslinkers Constructing Hydrogels with Stress-Relaxation and Bioactive Protein Modification诗人：Shi, Yanzhen Jing, Haowen Lu, Fanxuan Zhao, Mengying An, Shuiling Jin, Chang Gao, Yongdong Dai, Yinxin Zhu, Shuxu Yang, Songying Zhang, Xuesong Ye, Xiujun Cai, Yifan Wang期刊论文外链：

To evaluate the biological functionality of proteins loaded onto EV crosslinkers, we used VEGF as an example protein and encapsulated human umbilical vein endothelial cells (HUVECs) within SH-EV-VEGF crosslinked hydrogels (Figure 4g). After five days of culture, while HUVECs remained rounded and isolated within hydrogels crosslinked with 8-arm PEG-SH, the cells in EV-crosslinked hydrogels began to cluster, demonstrating that the stress relaxation properties of the EV-crosslinked hydrogels allowed cell movement and clustering (Figure 4h–j). Notably, the cell clusters were more prominent within SH-EV-VEGF crosslinked hydrogels. Interestingly, we observed a few instances of sprouting from the cell clusters in SH-EV-VEGF crosslinked hydrogels, indicating a trend toward angiogenesis (Figure 4h–j). In some cases, the sprouting almost connected two separate clusters, verifying that proteins loaded onto EV crosslinkers can maintain their biological function and influence the behavior of encapsulated cells.

只为测试加载失败到EV热塑剂上的蛋清质的海洋生物效果，企业食用VEGF做范本蛋清质，并将人脐门静脉内皮血细胞（HUVEC）包装在SH-EV-VEGF热塑水抑菌凝胶中。由三天的陪养，当HUVEC在与8臂PEG-SH交连的水抑菌抑菌妇科凝胶的作用中增加弧形和分割时，EV交连水抑菌抑菌妇科凝胶的作用中的细胞系膜膜逐渐积聚，是因为EV交连水抑菌抑菌妇科凝胶的作用的能力变松因素支持细胞系膜膜联通和积聚。非常值得考虑的是，SH-EV-EGF交连水抑菌抑菌妇科凝胶的作用中的细胞系膜膜簇更是为表现。人们观察分析到SH-EV-EGF化学热塑水疑胶中的体组织簇出显好几回些长芽现像，说明流血管转为的趋向。在某一前提下，长芽不错说联接了两个人同时的簇，查证了跳转到EV化学热塑剂上的蛋清质不错保护其动物基本功能并反应包封体组织的习惯。涉及到推见：Sulfo-Cy7-E 虎珀酰亚胺酯，855309-68-1cas:910482-46-1，Sulfo Cy5.5 NHS esterSulfo-Cy5.5-E 蜜腊酰亚胺酯，442912-55-2磷脂-聚乙二醇-N-乙酰半乳糖胺，cas:2936651-86-220379-59-3，β-D-吡喃红葡萄糖粉叠氮化物IRA410 Cl光敏树脂，9002-26-0246256-50-8，5(6)-羧基四甲基罗丹明特异性酯IR808双生物酯，IR808-NHS罗丹明标记图片甲基丙烯酰化明胶 GelMA