参考文献：运用共凝焦光学显微镜、dSTORM 和质谱新技术阐明与納米结构的脂质的载体在血脑深层穿线步骤中想关的蛋清质冠层的发展历程外部链接：//pubs.rsc.org/en/content/articlehtml/2022/nr/d2nr00484d小说作家：Matteo Battaglini ORCID ，Natalia Feiner bc， Christos Tapeinos a，Daniele De Pasquale a， Carlotta Pucci a，Attilio Marino a，Martina Bartolucci d，Andrea Petretto d，Lorenzo Albertazzi bc和 Gianni Ciofani 节选：原料和形式LMNV制法脂质磁体奈米质粒载体 (LMNV) 的加工意向书改编剧本自我国小组工作曾经的分析方案，依照了热高周波波和高压低压均质化 (HPH) 的办法。20简来说之，我国将不相同的脂质混合着一起来，及 2.5 mg 油酸（Sigma-Aldrich）、25 mg 1-硬脂酰-rac -甘油（Sigma-Aldrich）、2.5 mg 油酸（Sigma-Aldrich）、2.5 mg 1,2-二棕榈酰-rac-甘油-3-磷酸胆碱（Sigma-Aldrich）和 4 mg 1,2-二硬脂酰-sn-甘油-3-磷酸无水甲醇胺与共轭甲氧基聚乙二醇 (mPEG-DSPE) (5000 Da, Nanocs)，及 84.5 μl 超顺磁体阳极氧化铁奈米颗粒 (SPION) 的无水甲醇水溶液 (3 nm 长度，15 wt%；韩国分析方案奈米涂料我司) 复制到 6 ml 玻璃钢小瓶中。将3 ml加热（70 °C）的Tween® 80 (Sigma-Aldrich) 硫酸铜溶液 (1.0 wt%) 参与脂质/SPION解聚体中，并实用高周波波红外探头 (Fisherbrand™ Q125 Sonicator) 去高周波补救1五多15半个小时（波幅30%，电功率120 W）。高周波补救后，实用均质机以100 [细分隔符符（1/6-em）]000 psi的负担对融合物去各类高压变压器均质补救（共去5次各类高压变压器均质补救）。纳米技术技术膜蛋白在4 °C时以16 [细分隔符符（1/6-em）]000 g离心力式90多15半个小时（共去3次）去纯化，第三二次解聚于水内。要想去共凝聚显像，LMNV 实用荧光 Vybrant DiO 肿瘤细胞标注纺织染料（Invitrogen）去标注。将 5 mg 纳米技术技术膜蛋白与 20 μM DiO 在 37 °C 下孵育 2 每小时，第三在 4 °C 时以 16 [细分隔符符（1/6-em）]000 g离心力式 90 多15半个小时（俩次）去洗洁。而对于同时随意光学元件重塑显微镜观察 (dSTORM) 概述，在化学合成过程中中可能 3 mg mPEG-DSPE（5000 Da，Nanocs）与 1 mg DSPE-PEG-Cy3（5000 Da）融合去染色的。Materials and methodsLMNV preparationThe protocol for the fabrication of lipid magnetic nanovectors (LMNVs) was adapted from previous works of our group combining hot ultra-sonication and high-pressure homogenization (HPH) methods.20 Briefly, we mixed different lipids including 2.5 mg of oleic acid (Sigma-Aldrich), 25 mg of 1-stearoyl-rac-glycerol (Sigma-Aldrich), 2.5 mg of oleic acid (Sigma-Aldrich), 2.5 mg of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (Sigma-Aldrich), and 4 mg of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine with conjugated methoxyl poly(ethylene glycol) (mPEG-DSPE) (5000 Da, Nanocs) with 84.5 μl of an ethanol solution of superparamagnetic iron oxide nanoparticles (SPIONs) (3 nm diameter, 15 wt%; US Research Nanomaterials Inc.) into a 6 ml glass vial. 3 ml of pre-warmed (70 °C) Tween® 80 (Sigma-Aldrich) solution (1.0 wt%) were added to the lipid/SPION dispersion and sonicated using an ultrasonic tip (Fisherbrand™ Q125 Sonicator) for 15 min (amplitude 30%, 120 W). After the sonication, the mixture underwent high-pressure homogenization with a homogenizer at 100[thin space (1/6-em)]000 psi (5 passages of high-pressure homogenization were performed). The nanovectors were purified by centrifugation at 16[thin space (1/6-em)]000g for 90 min at 4 °C (three passages) and then re-dispersed in water. For confocal imaging, LMNVs were labeled with the fluorescent Vybrant DiO cell-labeling dye (Invitrogen) by incubating 5 mg of nanovectors with 20 μM of DiO for 2 h at 37 °C and then washing them by centrifugation at 16[thin space (1/6-em)]000g for 90 min at 4 °C (three passages). For Direct STochastic Optical Reconstruction Microscopy (dSTORM) analysis, the staining was obtained by mixing 3 mg of mPEG-DSPE (5000 Da, Nanocs) with 1 mg of DSPE-PEG-Cy3 (5000 Da) during the preparation procedure.

陕西pg电子娱乐游戏app 怪物提供了相关内容车辆：M2pep-PEG-DSPEEB1-PEG-DSPECPP-PEG-DSPECCK8-PEG-DSPEFSHB(QCHCGKCDSDSTDCT)-PEG-DSPEGRGDS-PEG-DSPEMMPs-PEG-DSPEWSW(WSWGPYS)-PEG-DSPELyP-1-PEG-DSPEVIP-PEG-DSPECREKA-PEG-DSPEAsp8-PEG-DSPE上面原创文章方面主要来源几大类期刊论文或资料，如果侵犯商标权请练习各位删除图片！