论文参考文献：联系共专注光学显微镜、dSTORM 和质谱系统蕴含与纳米级组成脂质平台在血脑障壁穿线的过程中关于的蛋白酶质冠层的演变史友链：//pubs.rsc.org/en/content/articlehtml/2022/nr/d2nr00484d诗人：Matteo Battaglini ORCID ，Natalia Feiner bc， Christos Tapeinos a，Daniele De Pasquale a， Carlotta Pucci a，Attilio Marino a，Martina Bartolucci d，Andrea Petretto d，Lorenzo Albertazzi bc和 Gianni Ciofani 节选：的材料和的方式LMNV制得脂质吸引力nm承载 (LMNV) 的生产加工合同样本改变自你们小组工作现在的探究，融合了热超声心动图波和油田均质化 (HPH) 方式。20简而言的之，你们将有所差异的脂质搭配在同食，也包括 2.5 mg 油酸（Sigma-Aldrich）、25 mg 1-硬脂酰-rac -甘油（Sigma-Aldrich）、2.5 mg 油酸（Sigma-Aldrich）、2.5 mg 1,2-二棕榈酰-rac-甘油-3-磷酸胆碱（Sigma-Aldrich）和 4 mg 1,2-二硬脂酰-sn-甘油-3-磷酸工业工业乙醇胺与共轭甲氧基聚乙二醇 (mPEG-DSPE) (5000 Da, Nanocs)，或 84.5 μl 超顺吸引力脱色铁nm阿尔法粒子 (SPION) 的工业工业乙醇液体 (3 nm 内直径，15 wt%；美国的探究nm的原材料有限公司) 倒进 6 ml 玻璃钢小瓶中。将3 ml加温（70 °C）的Tween® 80 (Sigma-Aldrich) 液体 (1.0 wt%) 引入脂质/SPION扩散体中，并用多普勒彩超波红外探头 (Fisherbrand™ Q125 Sonicator) 实行多普勒彩超处置1五分（波幅30%，最大功率120 W）。多普勒彩超处置后，用均质机以100 [细分隔符（1/6-em）]000 psi的水压对混和物实行油田低压均质处置（共实行5次油田低压均质处置）。微米技术各种膜蛋白在4 °C下,以16 [细分隔符（1/6-em）]000 g抽滤90分（共实行3次）实行纯化，然而呢自己扩散于水下。为了能实行共聚交三维成像，LMNV 用荧光 Vybrant DiO 受损细胞标注纺织染料（Invitrogen）实行标注。将 5 mg 微米技术各种膜蛋白与 20 μM DiO 在 37 °C 下孵育 2 20分钟，然而呢在 4 °C 下,以 16 [细分隔符（1/6-em）]000 g抽滤 90 分（三遍）实行洗衣。对于那些随时随即光电技术重新修建光学显微镜 (dSTORM) 解析，在制作工作中国上将 3 mg mPEG-DSPE（5000 Da，Nanocs）与 1 mg DSPE-PEG-Cy3（5000 Da）混和实行染色法。Materials and methodsLMNV preparationThe protocol for the fabrication of lipid magnetic nanovectors (LMNVs) was adapted from previous works of our group combining hot ultra-sonication and high-pressure homogenization (HPH) methods.20 Briefly, we mixed different lipids including 2.5 mg of oleic acid (Sigma-Aldrich), 25 mg of 1-stearoyl-rac-glycerol (Sigma-Aldrich), 2.5 mg of oleic acid (Sigma-Aldrich), 2.5 mg of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (Sigma-Aldrich), and 4 mg of 1,2-distearoyl-sn-glycero-3-phosphoethanolamine with conjugated methoxyl poly(ethylene glycol) (mPEG-DSPE) (5000 Da, Nanocs) with 84.5 μl of an ethanol solution of superparamagnetic iron oxide nanoparticles (SPIONs) (3 nm diameter, 15 wt%; US Research Nanomaterials Inc.) into a 6 ml glass vial. 3 ml of pre-warmed (70 °C) Tween® 80 (Sigma-Aldrich) solution (1.0 wt%) were added to the lipid/SPION dispersion and sonicated using an ultrasonic tip (Fisherbrand™ Q125 Sonicator) for 15 min (amplitude 30%, 120 W). After the sonication, the mixture underwent high-pressure homogenization with a homogenizer at 100[thin space (1/6-em)]000 psi (5 passages of high-pressure homogenization were performed). The nanovectors were purified by centrifugation at 16[thin space (1/6-em)]000g for 90 min at 4 °C (three passages) and then re-dispersed in water. For confocal imaging, LMNVs were labeled with the fluorescent Vybrant DiO cell-labeling dye (Invitrogen) by incubating 5 mg of nanovectors with 20 μM of DiO for 2 h at 37 °C and then washing them by centrifugation at 16[thin space (1/6-em)]000g for 90 min at 4 °C (three passages). For Direct STochastic Optical Reconstruction Microscopy (dSTORM) analysis, the staining was obtained by mixing 3 mg of mPEG-DSPE (5000 Da, Nanocs) with 1 mg of DSPE-PEG-Cy3 (5000 Da) during the preparation procedure.

西安市pg电子娱乐游戏app 动物展示 对应成品：M2pep-PEG-DSPEEB1-PEG-DSPECPP-PEG-DSPECCK8-PEG-DSPEFSHB(QCHCGKCDSDSTDCT)-PEG-DSPEGRGDS-PEG-DSPEMMPs-PEG-DSPEWSW(WSWGPYS)-PEG-DSPELyP-1-PEG-DSPEVIP-PEG-DSPECREKA-PEG-DSPEAsp8-PEG-DSPE及以上的文章的内容渠道各中文核心期刊或医学文献，告之图片侵权请认识我们大家误删！