DSPE-PEG-NBD及相关化学试剂采购与材料简介
参考文献:漆层化学活化核蛋白Anm*体偶联脂质体电机负载甲泼尼龙布挺高肺靶向药物非特异朋友及对发生急性肺挫裂伤的*成效微信链接://www.tandfonline.com/doi/full/10.1080/10717544.2017.1402217#abstract小说作者:李楠,东翁,王善梅,张元,陈珊珊,尹兆芳,斋嘉丽,朱迪·斯科布尔,夏洛特·C·威廉姆斯,陈涛,邱惠,秦五,赵可爱萌宠,卢立勤,泽维尔·穆莱特&李慧萍装修材料1,2-二硬脂酰-sn-甘油-3-磷酸胆碱 (DSPC)、硝基苯并呋咱 (DSPE-PEG-NBD) 购自 Avanti Polar Lipids, Inc. (阿拉巴马州阿拉巴斯特)。1,2-二硬脂酰-sn-甘油-3-磷酸无水乙醇胺-PEG 3400 -NH 2 (DSPE-PEG 3400 -NH 2 ) 购自 Nanocs Inc. (旧金山州旧金山市)。N-琥铂酰亚胺碘乙酸酯 (SIA 交连剂) 购自 Thermo Scientific (马萨诸塞州沃尔瑟姆)。磷酸二氢钠缓冲器液 (PBS, pH 7.4) 和胆固醇高 (CHOL) 购自 Sigma-Aldrich (密苏里州圣路易斯)。SP-A *理由南京优龙微食品科技产业比较有限司 (我们南京) 自动提炼。 SP-A納米*体(SPANb)由南京市肺科医生人工呼吸患病科学试验室自动提炼(Gajic等,引文2011)。几乎各个另外的化学反应品和石油醚均为阐述纯。几乎各个水相提纯均操作Milli-Q H 2 O (18.2 MΩ)。几乎各个有机化合物均擅自进这一步纯化操作。Materials1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC), nitrobenzofurazan (DSPE-PEG-NBD) was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL). 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-PEG3400-NH2 (DSPE-PEG3400-NH2) was purchased from Nanocs Inc. (New York, NY). N-Succinimidyl iodoacetate (SIA crosslinker) was purchased from Thermo Scientific (Waltham, MA). Phosphate buffered saline (PBS, pH 7.4) and cholesterol (CHOL) were purchased from Sigma-Aldrich (St. Louis, MO). SP-A antigen was synthesized by Shanghai YouLong Biotech Co., Ltd. (Shanghai, China). SP-A nanobody (SPANb) was synthesized by the Laboratory of Respiratory Disease, Shanghai Pulmonary Hospital, China (Gajic et al., Citation2011). All other chemicals and solvents were of analytical grade. Milli-Q H2O (18.2 MΩ) was used for all aqueous preparations. All compounds were used without further purification.