DSPE-PEG-DBCO介导的脂质体面功能表化与生物工程正构体制成连接：//www.researchgate.net/profile/Xiangdong-Xue-2/publication/390580878_Blocked_bioorthogonal_chemistry_enabled_switchable_bioorthosome_to_improve_liposomal_drug_delivery_for_glioblastoma_therapy/links/67f5bfb8e8041142a16fb0dc/Blocked-bioorthogonal-chemistry-enabled-switchable-bioorthosome-to-improve-liposomal-drug-delivery-for-glioblastoma-therapy.pdf原作者：Chao Wang, Jie Wu, Zhiran Duan, Yuqing Pan, Haijing Qu, Wei Cheng, Ning Wang, Han Chen, Xiaoli Gao,Mengqing Hou, Ying Zhang, and Xiangdong Xue节选：生物工程正构体的配制分为透明膜水合系统制作了海洋怪物正构体。重要讲，15毫克豆子的相混物磷脂酰胆碱（PC）、5毫克低密度胆固醇、0.6毫克DSPE-PEG2000，0.3mg DSPE-PEG-PBA和0.1mg DSPE-PEG-DBCO的相混物是充分光滑充分光滑溶解在甲醇/氯仿盐液体（1:3，v/v）中圆底烧瓶。操作旋转视频式汽化器汽化容剂汽化器在烧瓶内表皮建成薄脂质膜，那么实行负压晾干，以为了确保完完全全消除残余物容剂。得到脂质膜用4mL水复水在高周波波除理下，将含带2mg TMZ的PBS充分光滑充分光滑溶解。此然后呢将再水合的浮动液凭借11次操作Avanti Mini挤压出机的聚氨酯膜（200 nm）以获取光滑的脂质体。而后，MA（20毫克，0.06毫摩尔）将其成为脂质体盐液体中，那么在摇床周边孵育包夜以带动电无机化学体现这导至pH响应的性硼酸酯键（PBA-MA）的建成，然后导至然后海洋怪物正构体的人工。對照脂质体分为一样的的的方式实行人工，重要讲主要包括只含pH值的DBCO-MA脂质体现性和海洋怪物正交电无机化学屏蔽掉，和DBCO-PBA脂质体，其上缺乏性常见葡萄品种糖功用表皮。在身体外研发中包封罗丹明B（RhB），而吲哚青色（ICG）则用作休内整个脂质体组的海洋怪物分布范围定量分析。古诗网：Preparation of the BioorthosomeThe Bioorthosome was prepared using the thin-film hydrationtechnique. Specifically, a mixture of 15 mg soyphosphatidylcholines (PC), 5 mg cholesterol, 0.6 mg DSPE-PEG2000,0.3 mg DSPE-PEG-PBA, and 0.1 mg DSPE-PEG-DBCO wasdissolved in a methanol/chloroform solution (1:3, v/v) within around-bottom flask. The solvent was evaporated using a rotaryevaporator to form a thin lipid film on the flask's inner surface,followed by vacuum desiccation to ensure complete removal ofresidual solvents. The resulting lipid film was rehydrated with 4 mLof PBS containing 2 mg TMZ under ultrasonication. Therehydrated suspension was then passed 11 times through apolycarbonate membrane (200 nm) using an Avanti Mini-Extruderto obtain uniform liposomes. Thereafter, MA (20 mg, 0.06 mmol)was added to the liposome solution, which was subsequentlyincubated on a shaker overnight to promote the chemical reactionbetween PBA and MA. This resulted in the formation of pHresponsive borate ester bonds (PBA-MA), culminating in thesynthesis of the final Bioorthosome. Control liposomes weresynthesized employing the same methodology, specificallyincluding the DBCO-MA Lipo, which is devoid of pHresponsiveness and bioorthogonal chemistry shielding, as well asthe DBCO-PBA Lipo, which lacks glucose functionality on itssurface. For in vitro studies, rhodamine B (RhB) was encapsulated,while indocyanine green (ICG) was incorporated for in vivobiodistribution analysis across all liposome groups.

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