DSPE-PEG-NHS介导的CpG递送装置制定与免疫性可溶性好评友链：//link.springer.com/article/10.1007/s12257-020-0366-1著者：Jiwon Yang, Eun Seo Choi, Gayeon You & Hyejung Mok 绪论：由此可见CpG寡脱氧核苷酸（CpG）有整体实力强大的具有免役刺激性性用，開發技术CpG质粒媒体是实现了有效率癌症晚期具有免役*的先决情况。本分析将1,2-二硬脂酰-sn-甘油-3-磷酸工业乙醇胺-N-[羟基蜜腊酰亚胺（聚乙二醇）] (DSPE-PEG-NHS) 与聚乙稀亚胺 (PEI) 偶联，開發技术出那种PEI-PEG-DSPE偶联物，可用为食物混溶性的有效率CpG质粒媒体。我们公司開發技术了这五点PEIPEG-DSPE偶联物，每款偶联物的PEI原子核量差异，DSPEPEG的表达层面也差异，均表达出显著性性较低的生殖生殖受损体细胞核致毒。明确来讲，与依据具有PEI递送CpG好于，以摩尔比0.1递送PEI (25 kDa)-PEG-DSPE和DSPE-PEG-NHS/（PEI的胺基）可促使RAW264.7生殖生殖受损体细胞核对CpG的溶解挺高，这能够是在出现疏水溶性脂质部件。不仅而且，PEI-PEG-DSPE/CpGpp物可引导RAW264.7生殖生殖受损体细胞核显著性性外分泌生殖生殖受损体细胞核因素（TNF-α），其用与PEI/CpGpp物该是。以至于，PEI-PEG-DSPE偶联物可用为食物混溶性的有效率质粒媒体，将具有免役刺激性性剂CpG递送入巨噬生殖生殖受损体细胞核。译文翻译：Considering the potent immune stimulation by CpG oligodeoxynucleotides (CpGs), the development of CpG carriers is a prerequisite for efficient cancer immunotherapy. In this study, we conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[hydroxyl succinimidyl (polyethylene glycol)] (DSPE-PEG-NHS) with polyethylenimine (PEI) to develop a PEI-PEG-DSPE conjugate that can serve as a biocompatible and efficient CpG carrier. Five types of PEIPEG-DSPE conjugates were developed, each with different molecular weights of PEI and different degrees of DSPEPEG modification, and all exhibited significantly lower cytotoxicity. In particular, compared to CpG delivery via natural PEI, delivery with PEI (25 kDa)-PEG-DSPE and DSPE-PEG-NHS/(amine groups of PEI) at a molar ratio of 0.1 resulted in a higher uptake of CpGs into RAW264.7 cells, probably because of the presence of a hydrophobic lipid moiety. In addition, PEI-PEG-DSPE/CpG complexes triggered significant cytokine secretion (TNF-α) from RAW264.7 cells, comparable to that triggered by PEI/CpG complexes. Thus, PEI-PEG-DSPE conjugates could serve as biocompatible and efficient carriers of the immune stimulator CpG to the macrophages.

咸阳pg电子娱乐游戏app 生物制品供给关联设备：DSPE-PEG-VIP(二硬脂酰基磷脂酰甲醇胺-聚乙二醇-*血官特异性肠肽)DSPE-PEG-GE11(二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*人体细胞仁衣植物生长指数公式EGFR靶向治疗肽)DSPE-PEG-PTP(二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-胰腺癌靶向治疗肽)DSPE-PEG-FSHB(二硬脂酰基磷脂酰酒精胺-聚乙二醇-卵泡癌靶向药物肽)DSPE-PEG-R6H4（二硬脂酰基磷脂酰甲醇胺-聚乙二醇-PH初始化失败性体细胞穿膜肽)DSPE-PEG-SEARYL-R8（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-PH反应性组织穿膜肽)DSPE-PEG-SEARYL-PF6（二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-PH回复性人体细胞穿膜肽)DSPE-PEG-TH（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-PH没有响应性生殖细胞穿膜肽)DSPE-PEG-OTC（二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-种植抑索感觉靶向疗法肽)这些经典文章网站内容由来特殊杂志或论文参考文献，如遇抄袭请连接咱们去除！