DSPE-PEG-NHS介导的CpG递送制定制定与免疫抗体催化活性评测外链：//link.springer.com/article/10.1007/s12257-020-0366-1小说家：Jiwon Yang, Eun Seo Choi, Gayeon You & Hyejung Mok 前言：主要是因为CpG寡脱氧核苷酸（CpG）极具巨大的免役兴奋影响，发展管理CpG媒介是确保效率高化癌病免役*的先决的条件。本学习将1,2-二硬脂酰-sn-甘油-3-磷酸工业乙醇胺-N-[羟基虎珀酰亚胺（聚乙二醇）] (DSPE-PEG-NHS) 与聚氯乙烯亚胺 (PEI) 偶联，发展管理出一些PEI-PEG-DSPE偶联物，需用为食物混溶性的效率高化CpG媒介。咱们发展管理了这几种PEIPEG-DSPE偶联物，每样偶联物的PEI原子核量不同于，DSPEPEG的淡化阶段也不会同于，均表现形式出正相关较低的组织致癌性。重要来看，与采用具有PEI递送CpG相对来说，以摩尔比0.1递送PEI (25 kDa)-PEG-DSPE和DSPE-PEG-NHS/（PEI的胺基）可引起RAW264.7组织对CpG的降解更高的，这机会是主要是因为都存在疏水性聚氨酯脂质个部分。与此同时，PEI-PEG-DSPE/CpG挽回物可帮助RAW264.7组织正相关代谢组织细胞系因子（TNF-α），其影响与PEI/CpG挽回物一定。由于，PEI-PEG-DSPE偶联物需用为食物混溶性的效率高化媒介，将免役兴奋剂CpG递送进巨噬组织。译文翻译：Considering the potent immune stimulation by CpG oligodeoxynucleotides (CpGs), the development of CpG carriers is a prerequisite for efficient cancer immunotherapy. In this study, we conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[hydroxyl succinimidyl (polyethylene glycol)] (DSPE-PEG-NHS) with polyethylenimine (PEI) to develop a PEI-PEG-DSPE conjugate that can serve as a biocompatible and efficient CpG carrier. Five types of PEIPEG-DSPE conjugates were developed, each with different molecular weights of PEI and different degrees of DSPEPEG modification, and all exhibited significantly lower cytotoxicity. In particular, compared to CpG delivery via natural PEI, delivery with PEI (25 kDa)-PEG-DSPE and DSPE-PEG-NHS/(amine groups of PEI) at a molar ratio of 0.1 resulted in a higher uptake of CpGs into RAW264.7 cells, probably because of the presence of a hydrophobic lipid moiety. In addition, PEI-PEG-DSPE/CpG complexes triggered significant cytokine secretion (TNF-α) from RAW264.7 cells, comparable to that triggered by PEI/CpG complexes. Thus, PEI-PEG-DSPE conjugates could serve as biocompatible and efficient carriers of the immune stimulator CpG to the macrophages.

浙江pg电子娱乐游戏app 生物工程带来了一些成品：DSPE-PEG-VIP(二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-*血管壁生物肠肽)DSPE-PEG-GE11(二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*细胞核上皮组织发展指数公式EGFR靶向治疗肽)DSPE-PEG-PTP(二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-胰腺癌靶向治疗肽)DSPE-PEG-FSHB(二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-暖巢癌靶点肽)DSPE-PEG-R6H4（二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-PH崩溃性肿瘤细胞穿膜肽)DSPE-PEG-SEARYL-R8（二硬脂酰基磷脂酰无水乙醇胺-聚乙二醇-PH没有响应性肿瘤细胞穿膜肽)DSPE-PEG-SEARYL-PF6（二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-PH加载性受损细胞穿膜肽)DSPE-PEG-TH（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-PH反映性人体细胞穿膜肽)DSPE-PEG-OTC（二硬脂酰基磷脂酰甲醇胺-聚乙二醇-种子发芽抑索肾上腺素受体靶向治疗肽)及以上句子东西种类当下论文期刊或论文参考文献，予以侵权案请练习公司移除！