您当前所在位置:首页 > 宣传资料 > 其他试剂
PLL-PEG-PLL构建的PIC胶束用于DNA递送
发布时间:2025-07-24     作者:kx   分享到:
论文资料:PLL-PEG-PLL的光催化原理还有在DNA封装形式中的技术应用跳转://link.springer.com/article/10.1007/s11426-006-2006-9编辑:杨子刚,周芬,袁建军,马立新,翟超&程士元节选:经由对PLL(Z)-PEG-PLL(Z)中氨基做好脱养护,合并了PLL-PEG-PLL。探究了PLL-PEG-PLL与DNA变成的聚化合物塑料物(PIC)胶束的属性。结论表面,PIC的重点是由PLL与DNA经由电磁干扰相互之间功能变成的,PEG为PIC的的外壳,由此PIC胶束在水相如表匀区域划分,所有在弱酸性状态下就不会放置,具备有正常的稳相关性性,这在人类基因*中具备有决定性实际意义。的同时,结论表面PLL-PEG-PLL会对DNA做好缩合,且PLL嵌段越长,缩合郊果更好,有时候DNA的*核酸酶几丁质酶也明显提生。PIC呈圆球形,长度为10~35 nm,方便于被細胞摄入。身体外转染实验报告表面,所包装的DNA会在SF9細胞系中理解。

PLL-PEG-PLL

PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10–35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.银川pg电子娱乐游戏app 生物技术打造相关新产品:PLL-g-PEG-BiotinPLL-g-PEG-N3PLL-PEGPLL-PEG-N3PLL-PEG-OPSSPoly(L-Aspartic acid)-PEGsPoly(L-Ornithine)-MPEG上述文章内部内部原因各种各样期刊论文或专著,深表歉意图片侵权请联络我门刪除!