您当前所在位置:首页 > 宣传资料 > 其他试剂
PLL-PEG-PLL构建的PIC胶束用于DNA递送
发布时间:2025-07-24     作者:kx   分享到:
资料:PLL-PEG-PLL的化学合成名词解释在DNA芯片封装中的应运联接://link.springer.com/article/10.1007/s11426-006-2006-9小说家:杨子刚,周芬,袁建军,马立新,翟超&程士元节选:按照对PLL(Z)-PEG-PLL(Z)中氨基完成脱庇护,合成视频了PLL-PEG-PLL。学习了PLL-PEG-PLL与DNA达成的聚铁离子pp物(PIC)胶束的特点。的成果反映出,PIC的层面是由PLL与DNA按照防静电互不功能达成的,PEG为PIC的底壳,但是PIC胶束在水相中其匀区域,如果在一般的中性环境下也会沉淀物中,都都具有较好的稳固性,这在遗传基因*中都都具有比较重要目的意义。此外,的成果反映出PLL-PEG-PLL能够对DNA完成缩合,且PLL嵌段越长,缩合使用效果数越,和DNA的*核酸酶化学活化也有明显延长。PIC呈圆球形,直径约为10~35 nm,适于被上皮组织细胞摄食。身体转染实验设计反映出,所包的DNA能够在SF9上皮组织细胞系中表示。

PLL-PEG-PLL

PLL-PEG-PLL was synthesized by deprotection of the amino group of PLL(Z)-PEG-PLL(Z). The properties of the polyion complex (PIC) micelles comprising of PLL-PEG-PLL and DNA were investigated. The results showed that the core of PIC was formed by PLL and DNA through electrostatic interactions, and PEG was shell of PIC, so PIC micelles in aqueous medium were homogenous with no precipitation even under the neutral condition, and PIC had the characteristic of stability, which were very important in gene therapy. At the same time, the results showed PLL-PEG-PLL could condense DNA, longer PLL blocks were generally more effective at condensation, and the nuclease resistance of DNA was increased remarkably. PIC was spherical with diameter of 10–35 nm, which could be easily taken up by cells. Transfection experiments in vitro showed that the DNA encapsulated could be expressed in the cell line SF9.石家庄pg电子娱乐游戏app 生物学展示 涉及成品:PLL-g-PEG-BiotinPLL-g-PEG-N3PLL-PEGPLL-PEG-N3PLL-PEG-OPSSPoly(L-Aspartic acid)-PEGsPoly(L-Ornithine)-MPEG之内一篇文章信息内容因素各项杂志期刊或学术论文,予以知识产权侵权请关系人们误删!