论文参考文献：Tumor angiogenesis targeting and imaging using gold nanoparticle probe with directly conjugated cyclic NGR†做者：Minghao Wu‡ ORCID logoa, Yanyan Zhang‡a, Ying Zhanga, Mingjie Wub, Menglin Wua, Hongyi Wua, Lin Caoa, Liang Lia, Xue Li*a and Xuening Zhang*a论文外部链接：//pubs.rsc.org/en/content/articlehtml/2018/ra/c7ra10155d文献综述：Preparation of FITC coupled GNPs–PEG@cNGR and GNPs–PEG.To prepare FITC coupled GNPs–PEG@cNGR and GNPs–PEG, 1 mg of the GNPs–PEG@cNGR probe, previously synthesized, was dispersed into 1 mL crosslinking reaction solution containing 7.56 mg Na2CO3, 1.06 mg NaHCO3, and 7.36 mg NaCl, sonicated for 2 min, and stored at 4 °C for 10 min. Subsequently, 0.3 mg FITC in 300 μL of DMSO was poured into the above solution under gentle and continuous stirring and incubated in the dark for 8 h at 4 °C. Next, 20 μL NH4Cl solution (5 M) was poured into the above solution under gentle and continuous stirring to terminate the above reaction and then incubated in the dark for 2 h at 4 °C. The final GNPs–PEG@cNGR@FITC was retrieved by ultrafiltration with a Millipore centrifugal filter unit (Amicon Ultra-0.5; MWCO 100[thin space (1/6-em)]000), washed three times, and resuspended in 0.5 mL of PBS (pH 7.4).In addition, FITC-conjugated GNPs–PEG (GNPs–PEG@FITC) was also prepared as a control. Briefly, the preparation was similar to the fabrication of GNPs–PEG@cNGR, but 220 μL SH–cNGR (1 mg mL−1) was replaced by 15 μL 2-aminoethanethiol (20 mM).

準備FITC藕合的GNP——PEG@cNGR与GNP-PEG，1毫克GNP-PEGPEG@cNGR将前一天分解成的电极细化到包含有7.56mg Na2CO3、1.06mg NaHCO3和7.36mg NaCl的1mL交连化学反应悬浊液中，彩超补救2多分钟的英文，在4°C下存放10多分钟的英文。自后，在轻柔重复混合下，将0.3mg FITC不能溶解300μL DMSO中倒进去上面的溶剂中，在4℃下背光孵育8个钟头。现在来，在轻柔重复混合下将20μL NH4Cl溶剂（5M）倒进去上面的溶剂中以中止上面的化学反应，第二步在4°C下到黑暗里孵育2个钟头。决定的GNPPEG@cNGR@操作Millipore离心力过滤清洁器单无（Amicon Ultra-0.5；MWCO 100[薄发展空间（1/6-em）]000）确定超滤膜，收售FITC，干洗六次，原则新透明桌面在0.5 mL PBS（pH 7.4）中。然而，FITC偶联的GNP-PEG（GNP-PEG）PEG@FITC)也做好准备做相较比较。简一般来说之，化学合成的过程 近似于于GNP的产生——PEG@cNGR，但220μL SH-cNGR（1 mg mL-1）被15μL 2-氨基乙硫醇（20 mM）所改变。对应举荐：Biotin-PEG-PFPDiethoxylsilane-PEG-BiotinMonoethoxylsilane-PEG-BiotinBiotin-PEG-TretinoinNTA-PEG-BiotinBiotin-PEG-SSBiotin-PEG-SGBiotin-PEG-HZBiotin-PEG-SVABiotin-PEG-ACBiotin-PEG-CHODBCO-PEG-BiotinBiotin-PEG-FA综上所述篇文章游戏内容渠道各杂志期刊或期刊论文，假如商标侵权请链接你们删出！