论文资料：Evaluation of the physicochemical properties and the biocompatibility of polyethylene glycol-conjugated gold nanoparticles: A formulation strategy for siRNA delivery小说作者：Kamil Rahme a b c 1, Jianfeng Guo d 1, Justin D. Holmes a b, Caitriona M. O’Driscoll论文超链接：论文摘要：Recently, the potential of gold nanoparticles (AuNPs) for transporting drugs, proteins and genetic materials has been demonstrated. Previously, our laboratory synthesised positively charged, surfactant-free AuNPs in water by the reduction of gold (III) chloride (AuCl3) using hydroxylamine hydrochloride (NH2OH·HCl) in the presence of l-cysteine methyl ester hydrochloride (HSCH2CH(NH2)COOCH3·HCl) as a capping agent. These AuNPs, which achieve higher cell viability in comparison to cetyl trimethyl ammonium bromide (CTAB, a surfactant)-capped counterparts, have demonstrated potential for siRNA delivery. However, it is well known that systemic administration of cationic delivery systems without biological stablising moieties causes non-specific binding with negatively charged serum proteins, resulting in particle aggregation and opsonisation. Consequently, highly stable AuNPs capped with l-cysteine methyl ester hydrochloride conjugated to poly(ethylene glycol) (PEG) were synthesised in this study. PEGylation enhanced the biocompatibility of the AuNPs by reducing toxicity in a range of cell types, by inhibiting interaction with serum proteins thus avoiding aggregation, and, by providing protection against degradation by nucleases. Moreover, these PEGylated AuNPs formed nanoparticles (NPs) with siRNA (which was first compacted with protamine), and had a diameter within the nanoscale range (∼250 nm) and a near neutral surface charge (∼10 mV). In the future a bifunctional PEG chain on the AuNPs (i.e., SH-PEG-NH2, SH-PEG-COOH) will be used to facilitate conjugation of a targeting ligand to enhance cell specific uptake.

金微米再生颗粒（AuNP）在运载药材、淀粉酶质和基因有机化合物这方面的潜质获取了表明。半年前，我的科学试验室在l-半胱氨酸甲酯酸洗盐（HSCH2CH（NH2）COOCH3·HCl）是 封端剂的有着下，用到酸洗羟胺（NH2OH·HCl）展现氯化金（III）（AuCl3），在池中人工带正带电粒子、无外观亲水性剂的AuNP。与第十六烷基前三基溴化铵（CTAB，另外一种外观抗逆性剂）封新风系统的表示物不同于，以上AuNP含有极高的上皮细胞成活率，现已材料了siRNA递送的潜力股。既使，沒有生物技术比较稳定性高一些的阳铁离子递送体系的下半身给药会会会造成与带负电荷量的血清核蛋白的非特情人融入，以此会会造成顆粒聚在一起和调理身体。但是，本探讨制成了用与聚乙二醇（PEG）共轭的l-半胱氨酸甲酯稀盐酸盐封中端高强度比较稳定性高的AuNP。聚乙二醇化按照缩减一系统细胞系类型、的致毒、减弱与血清淀粉酶的相互间意义为了以防集聚，已经保证以防核酸酶怪物降解的护理，激发了AuNP的怪物混溶性。还有，以上聚乙二醇化的AuNP与siRNA（首要用鱼精淀粉酶级配碎石）进行微米科粒（NP），厚度在微米级规模内（约250 nm），外表面带电粒子亲近中性化（约10 mV）。的前景，AuNP上的双职能PEG链（即SH-PEG-NH2、SH-PEG-COOH）将用作提高靶向疗法配体的偶联，以增強体细胞特女性朋友摄入。相应分享：Biotin-PEG-FABiotin-PEG-NHSAlkyne-PEG-BiotinSilane-PEG-BiotinLA-PEG-BiotinIA-PEG-BiotinBiotin-PEG-ACAN3-PEG-BiotinOPSS-PEG-BiotinBiotin-PEG-MalBiotin-PEG-SCMSH-PEG-BiotinBiotin-PEG-OH之上的文章方面来源于各种各样刊物或学术论文，请谅解侵犯著作权请联络我国删了！