期刊论文：Endocytic Mechanisms of Graphene Oxide Nanosheets in Osteoblasts, Hepatocytes and Macrophages原作者：Javier Linares†M. Concepción Matesanz†Mercedes Vila‡§⊥M. José Feito†Gil Gonçalves⊥María Vallet-Reg퇧Paula A. A. P. Marques⊥M. Teresa Portolés论文参考文献环节： 内容提要：Nano-graphene oxide (GO) has attracted great interest in nanomedicine due to its own intrinsic properties and its possible biomedical applications such as drug delivery, tissue engineering and hyperthermia cancer therapy. However, the toxicity of GO nanosheets is not yet well-known and it is necessary to understand its entry mechanisms into mammalian cells in order to avoid cell damage and human toxicity. In the present study, the cellular uptake of pegylated GO nanosheets of ca. 100 nm labeled with fluorescein isothiocyanate (FITC-PEG-GOs) has been evaluated in the presence of eight inhibitors (colchicine, wortmannin, amiloride, cytochalasin B, cytochalasin D, genistein, phenylarsine oxide and chlorpromazine) that specifically affect different endocytosis mechanisms. Three cell types were chosen for this study: human Saos-2 osteoblasts, human HepG2 hepatocytes and murine RAW-264.7 macrophages. The results show that different mechanisms take part in FITC-PEG-GOs uptake, depending on the characteristics of each cell type. However, macropinocytosis seems to be a general internalization process in the three cell lines analyzed. Besides macropinocytosis, FITC-PEG-GOs can enter through pathways dependent on microtubules in Saos-2 osteoblasts, and through clathrin-dependent mechanisms in HepG2 hepatocytes and RAW-264.7 macrophages. HepG2 cells can also phagocytize FITC-PEG-GOs. These findings help to understand the interactions at the interface of GO nanosheets and mammalian cells and must be considered in further studies focused on their use for biomedical applications.

nm技术氧化物苯（GO）是因为其之前的概念基本在药物剂量输送机、结构建筑工程和热疗等菌物中医药学科技领域的潜在性技术应用，吸引了顾客对nm技术中医药学的很大程度上业余爱好。同时，GOnm技术片的致癌性尚不清除，有需要介绍其流入喂奶节肢动物肿瘤细胞核的共识机制，以防止肿瘤细胞核挫裂伤和躯干致癌性。在本研发中，在几种缓和剂（秋月仙素、渥曼宁、阿米洛利、人体体癌细胞松驰素B、人体体癌细胞松驰球蛋白D、有机染料木素、苯胂氧化的物和氯丙嗪）的产生下，分析了用异硫氰酸荧光素（FITC-PEG-GO）标记符号的约100nm的聚乙二醇化GO納米片的人体体癌细胞摄入，这么多缓和剂正规会影响不同于的内吞缘由。本研发选购了三个血生殖受损细胞核分类：人Saos-2成骨血生殖受损细胞核、人HepG2肝血生殖受损细胞核和小鼠RAW-264.7巨噬血生殖受损细胞核。但是揭示，表明每一种的神经元膜类形的功能，各种不同的制度流入了FITC-PEG-GOs的摄入。可是，在所讲解的六种神经元膜系中，大顆粒神经元膜出现依然是一个个大都的内化过程中。不光巨噬神经元膜效用外，FITC-PEG-GOs还可能按照Saos-2成骨神经元膜中依赖关系于微管的有效途径流入，并按照HepG2肝神经元膜和RAW-264.7巨噬神经元膜中的网格蛋白酶依赖关系于制度流入。HepG2组织也不错吞灭FITC-PEG-GOs。此类表明可进1步看待GO纳米级片和蒲乳期两栖动物组织接面上的彼此用，在进1步科研其在生物体临床APP软件中的APP软件时须得恰当充分考虑。重要性高性价比：FITC-PEG-DMGFITC-PEG-FAFITC-PEG-BiotinOH-PEG-FAMNH2-PEG-FAMNTA-PEG-FITCFAM-PEG-N3FITC-PEG-ACFITC-PEG-ACAFITC-PEG-AlkyneFITC-PEG-AzithromycinFITC-PEG-CHOFITC-PEG-DTPAFITC-PEG-Estrogen以上的新闻稿件方式种类各大期刊论文或学术论文，要是有侵犯著作权请关联他们清空！