参考文献：Endocytic Mechanisms of Graphene Oxide Nanosheets in Osteoblasts, Hepatocytes and Macrophages我们：Javier Linares†M. Concepción Matesanz†Mercedes Vila‡§⊥M. José Feito†Gil Gonçalves⊥María Vallet-Reg퇧Paula A. A. P. Marques⊥M. Teresa Portolés论文参考文献链接代码： 结语：Nano-graphene oxide (GO) has attracted great interest in nanomedicine due to its own intrinsic properties and its possible biomedical applications such as drug delivery, tissue engineering and hyperthermia cancer therapy. However, the toxicity of GO nanosheets is not yet well-known and it is necessary to understand its entry mechanisms into mammalian cells in order to avoid cell damage and human toxicity. In the present study, the cellular uptake of pegylated GO nanosheets of ca. 100 nm labeled with fluorescein isothiocyanate (FITC-PEG-GOs) has been evaluated in the presence of eight inhibitors (colchicine, wortmannin, amiloride, cytochalasin B, cytochalasin D, genistein, phenylarsine oxide and chlorpromazine) that specifically affect different endocytosis mechanisms. Three cell types were chosen for this study: human Saos-2 osteoblasts, human HepG2 hepatocytes and murine RAW-264.7 macrophages. The results show that different mechanisms take part in FITC-PEG-GOs uptake, depending on the characteristics of each cell type. However, macropinocytosis seems to be a general internalization process in the three cell lines analyzed. Besides macropinocytosis, FITC-PEG-GOs can enter through pathways dependent on microtubules in Saos-2 osteoblasts, and through clathrin-dependent mechanisms in HepG2 hepatocytes and RAW-264.7 macrophages. HepG2 cells can also phagocytize FITC-PEG-GOs. These findings help to understand the interactions at the interface of GO nanosheets and mammalian cells and must be considered in further studies focused on their use for biomedical applications.

納米空气氧化苯（GO）基于其僵板的概念名词解释在中成药输料、机构施工和热疗等怪物医疗业务领域的潜在性运用，致使了大众对納米医疗的有效好奇心。不过，GO納米片的致毒尚不很清楚，有需要了解到其渗入喂母乳动物界体癌细胞的机能，以应对体癌细胞磨损和人身体致毒。在本学习中，在几种可以抑溶液剂（潇潇暮雨仙素、渥曼宁、阿米洛利、癌生殖肿瘤细胞松散素B、癌生殖肿瘤细胞松散蛋清D、有机染料木素、苯胂氧化反应物和氯丙嗪）的会出现下，评估方法了用异硫氰酸荧光素（FITC-PEG-GO）标记图片的约100nm的聚乙二醇化GO微米片的癌生殖肿瘤细胞摄取量，他们可以抑溶液剂特地关系有所不同的内吞策略。本学习选泽了四种癌人体血细胞系结构类型：人Saos-2成骨癌人体血细胞系、人HepG2肝癌人体血细胞系和小鼠RAW-264.7巨噬癌人体血细胞系。导致得出结论，依照多种有差异血生殖神经元系类的的特点，有差异的体系进行了FITC-PEG-GOs的摄入。殊不知，在所数据分析的三个血生殖神经元系系中，大粒子血生殖神经元系不断增多也许也是个非常的内化的过程。代替巨噬血生殖神经元系的作用外，FITC-PEG-GOs还是可以经过Saos-2成骨血生殖神经元系中依赖关系感微管的渠道来到，并经过HepG2肝血生殖神经元系和RAW-264.7巨噬血生殖神经元系中的网格蛋白质依赖关系感体系来到。HepG2体组织也都可以摧毁FITC-PEG-GOs。以下发展这会有利于领悟GO纳米技术片和喂乳两栖动物体组织工具栏上的互不用，在进一歩学习其在海洋生物药学操作中的操作时不得不充分了解。有关于分享：FITC-PEG-DMGFITC-PEG-FAFITC-PEG-BiotinOH-PEG-FAMNH2-PEG-FAMNTA-PEG-FITCFAM-PEG-N3FITC-PEG-ACFITC-PEG-ACAFITC-PEG-AlkyneFITC-PEG-AzithromycinFITC-PEG-CHOFITC-PEG-DTPAFITC-PEG-Estrogen不低于原创文章网站内容源于多种刊物或论文，假如有版权侵权请结合企业删了！