论文参考文献：Folate-mediated tumor cell targeting of liposome-entrapped doxorubicin in vitro创作者：Robert J Lee, Philip S Low资料外部链接： 小结：Receptors for the vitamin folic acid are frequently overexpressed on epithelial cancer cells. To examine whether this overexpression might be exploited to specifically deliver liposome-encapsulated drug molecules in vitro, folate-targeted liposomes were prepared by incorporating 0.1 mol% of a folate-polyethyleneglycol-distearoylphosphatidylethanolamine (folate-PEG-DSPE) construct into the lipid bilayer, and were loaded with doxorubicin (DOX), an anti-cancer drug. Uptake of folate-PEG-liposomal DOX by KB cells was 45-fold higher than that of non-targeted liposomal DOX, and 1.6-times higher than that of free DOX, while the cytotoxicity was 86 and 2.7-times higher, respectively. Folate-targeting is fully compatible with PEG-coating of the liposomes, since incorporation of 4 mol% PEG2000-DSPE does not reduce the uptake or cytotoxicity of folate-PEG-liposomal DOX. Uptake of folate-PEG-liposomes was inhibited by 1 mM free folic acid but was unaffected by physiological concentrations of folate. In HeLa/W138 co-cultures, folate-PEG-liposomes encapsulating calcein, a fluorescent dye, were found to be almost exclusively internalized by the HeLa cells which overexpress the folate receptors. We suggest that folate targeting constitutes a possible mechanism for improving the specificity of PEG-coated liposomes for cancer cells.

全面检查这个无限表达方式是不是也可以于在身体特异形递送脂质体邮包的类药物剂量氧分子，经由将0.1mol%的孕妇DHA片-亚乙基二醇-二硬脂酰磷脂酰甲醇胺（孕妇DHA片-PEG-DSPE）共建体加入适量脂质两层中准备孕妇DHA片靶向疗法脂质体，并过载类药物剂量阿霉素（DOX）。KB细胞系膜对孕妇叶酸-PEG脂质体DOX的摄入量比非靶向药物DOX高45倍，比游走DOX高1.6倍，而细胞系膜渗透性分別高86倍和2.7倍。DHA靶点与脂质体的PEG表层完完全全兼容，鉴于掺加4 mol%的PEG2000-DSPE并不会下降DHA-PEG脂质体DOX的摄食或内部致毒。备孕备孕叶酸片-PEG脂质体的摄入感受到1mM徘徊备孕备孕叶酸片的治理和改善，但不主备孕备孕叶酸片人体生理氧浓度的应响。在HeLa/W138共训练中，会发现包封钙黄绿素（一些荧光染色剂）的备孕叶酸片-PEG脂质体基本上根本被过展示备孕叶酸片多巴胺受体的HeLa細胞内化。涉及到强烈推荐：AF 647 NHS ester113721-87-2，AMCA-OSuCoroNaGreen129423-53-6，SBFI AMAF430 carboxylic acid，Alexa Fluor430 COOH852953-60-7，AF405-acidAF350 NHS荧光有机染料AF488 carboxylic acidAF555NHS esterCyanine3-(OH)2，Cy3-(OH)2上文原创文章东西主要来源当下学术期刊或论文参考文献，烦请侵犯商标权请认识你们删除文件！