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DSPE-PEG2000-NH₂修饰构建AFP靶向磁性荧光脂质体探针
发布时间:2025-07-15     作者:kx   分享到:
参考文献:甲胎核蛋白*体遮盖荧光吸引力探头对HepG2体细胞及*型号小鼠的 探讨链接转换://www.ingentaconnect.com/contentone/asp/jnn/2020/00000020/00000008/art00088小说作家:陈艳帆,卢江环,杨杰,郝凯旋,李敏节选:为融合有的是种双靶点治疗(磁靶点治疗和*体靶点治疗)便携式键带磁荧光脂质体探头,该探头具正常的海洋生物工程混溶性,对之前肝*的原因具较高的炎症因子聊天。称取1,2-二硬脂酰-sn-甘油-3-磷酸-乙酸乙酯胺-N- [氨基(聚乙二醇)](DSPE-PEG2000-NH2)与Cy7 N-羟基虎珀酰亚胺酯(Cy7-NHS)实施生理不起作用,转换成DSPE-PEG 2000 -Cy7。将鸡蛋黄卵磷脂、胆固醇升高、DSPE-PEG 2000 -Cy7和Fe 3 O 4分别下载到生理不起作用液中,结合荧光四硫化三铁(Fe 3 O 4)脂质体。将带磁脂质体(MLP)与甲胎球蛋白(AFP)多克隆*体偶联,并对末梢AFP*体修饰语的带磁脂质体在人肝*组织HepG2和肝*型号小鼠中的用实施了讲解深入分析。荧光四硫化三铁脂质体呈球型,年均流体力学性原因学直径怎么算约150nm,带负正电荷。该荧光带磁探头对HepG2组织毒副作用较小,并不断提高了AFP*体靶点治疗影像在肝*型号小鼠肝*原因中的炎症因子聊天。总的来说,AFP*体偶联的荧光带磁探头是有的是种很有前程的便携式键软件,具正常的海洋生物工程混溶性,并具较高的迅敏度和炎症因子聊天,要用于临床医学*症的原因和*。

DSPE-PEG2000-NH₂

To construct a dual-targeting (magnetic targeting and antibody targeting) multi-functional magnetic fluorescent liposome probe with a good biocompatibility and high specificity for early liver cancerdiagnosis. 1,2-distearoyl-sn-glycero-3-phospho-ethanolamine-N-[amino(polyethylene glycol)] (DSPE-PEG2000-NH2) and Cyanine7 N-hydroxysuccinimide ester (Cy7-NHS) were measured and reacted for forming DSPE-PEG2000-Cy7. Egg yolk lecithin, cholesterol, DSPE-PEG2000-Cy7 and Fe3O4 were added in orderly into the reaction solution for synthesis of fluorescent ferroferric oxide (Fe3O4) liposome. The Magnetic liposome (MLP) was coupled with Alpha-Fetoprotein (AFP) polyclonal antibody and the ending AFP antibody modified magnetic liposome was analyzed and studied on human hepatoma cell HepG2 and cancer model mouse. The fluorescent ferroferric oxide liposome appeared as a sphericalmorphology and they were about 150 nm in mean hydrodynamic diameter with negative charge. The fluorescent magnetic probe had a little toxic effect on hepG2 cells and increased the specificity liver cancer diagnosis by AFP antibody target imaging in model mouse of liver cancer. In brief, the AFP antibody conjugated fluorescent magnetic probe is a promising multi-functional tool with good biocompatibility and high sensitivity and specificity for clinical cancer diagnosis and therapy.宝鸡pg电子娱乐游戏app 生物学可以提供相关产品的:DSPE-PEG-MalDSPE-RBDSPE-PEI-DextranLyP-1-PEG-DSPEAngiopep-2 PEGDSPE-PEG-CSNRDARRC(二硬脂酰基磷脂酰甲醇胺-聚乙二醇-*靶向治疗淀粉酶)DSPE-PEG-BSA及以上文章标题知识因素各项学术期刊或学术论文,若有图片侵权请认识人们去除!