SH-PEG-COOH修饰金纳米棒的合成及多模态信号增强应用研究
医学文献:Surface Functionalized Gold Nanorods: Tracking and Observing Live Cell via Three Optical Signals 小说家:Qiu, Xiaozhong; Wang, Yaling; Cui, Yanyan; Sun, Zhipeng; Liu, Ru; Huang, Wenhua; Zhao, Feng; Zu, Yan; Yuan, Hui; Chang, Xueling; Gao, and Xueyun学术论文联接: Surface functionalized gold nanorods (GNRs) with three optical signals and the ability to track and observe live cells was investigated. The Cetyltrimethyl Ammonium Bromide (CTAB) was used as the shape-directing agent to develop GNRs with uniform size and aspect ratio. The as-synthesized GNRs can strongly enhance the Raman signal of the attached MGITC on the GNRs. Polyethylene glycol (SH-PEG-COOH) and SH-PEG-FITC were attached with GNRs through ligand exchange with CTAB. They can stabilize the GNRs, reduce the cytotoxicity and provide fluorescence signal. A cell-penetrating peptide was covalently conjugated with SH-PEG-COOH to assist the GNRs to enter living cell. After cultured with the MCF-7 cells, the surfaced functionalized Au nanorods can enter the MCF-7 cells and be tracking with enhanced MGITC Raman signal, FITC fluorescence, and Rayleigh scatter signal.