参考文献：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†编辑：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song文章连接：文献综述：The NPs were prepared according to a previously published procedure.33 Briefly, 50 mg of TPP–PEG–biotin was dissolved in 5 ml of chloroform, dried under a flow of nitrogen gas, and dried further under vacuum to get a film layer. For nanoparticle synthesis, the TPP–PEG–biotin film layer was hydrated with 900 μl of PBS (pH 7.4) and 100 μl of DMSO. Then the solution mixture was sonicated for 15 min, and uniform sized self-assembled TPP–PEG–biotin NPs were obtained slowly. The NPs were collected by centrifugation at 15[thin space (1/6-em)]000 rpm using a TJ-25 centrifuge (Beckman Coulter). The characterization of TPP–PEG–biotin SANs was done by TEM and SEM. The stock was diluted with PBS (pH 7.4) and used for in vitro experiments.

TPP-PEG-海洋生物素自折装纳米级阿尔法粒子的制取NP是只能根据先前发布公告的流程备制的。将50 mg TPP–PEG–动物素溶于在5 ml氯仿中，在氢气落下缺水，如果在正空下进一个步骤缺水以拿到膜层。我们对纳米技术物体制作而成，TPP-PEG-微生物技术素膜层用900μl PBS（pH 7.4）和100μl DMSO水合。最后将溶剂组合物物高周波进行处理1五一分钟，迟缓提升透亮大小不一的自安装TPP-PEG-微生物技术素NP。食用TJ-25抽滤机（Beckman Coulter）以15[薄办公空间（1/6-em）]000rpm的高速度抽滤收集整理NP。使用散射光微电子透射电镜（TEM）和打印机扫描光微电子透射电镜（SEM）对TPP-PEG-生物技术素-SAN开展了定量分析。将原液用PBS（pH 7.4）溶解稀释，代替身体进行实验。有关系推薦：SC-PEG-CH2CH2COOtBuSC-PEG-CLASC-PEG-DEPSC-PEG-MTASC-PEG-OLASC-PEG-PLA(2K)SC-PEG-TretinoinSH-PEG-SCSilane-PEG-SCSTA-PEG-SCTocopherol-PEG-SCTocopherol-PEG-SCMA-PEG-MA CAS No.: 25852-47-5SAA-PEG-SAAGAA-PEG-GAASA-PEG-SAGA-PEG-GA上文软文的内容的来源种类期刊杂志或资料，告之侵犯肖像权请保持联系.我去除！