您当前所在位置:首页 > 资讯信息 > 新品上市
TPP-PEG-biotin的合成
发布时间:2025-07-15     作者:zyl   分享到:
论文参考文献:Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†诗人:Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song文章微信链接: 前言: The TPP–PEG–biotin chemical was synthesized by the acid–amine coupling reaction between biotin–PEG–COOH and TPP-amine. Briefly, 1 mmol of biotin–PEG–COOH, DMAP, EDCI and 3 ml of CH2Cl2 were added to a glass vial and the reaction mixture stirred for 3 h at 0 °C. Then 1 mmol of TPP-NH2 was added and the reaction mixture was stirred continuously for 36 h at room temperature under a N2 atmosphere. The unconjugated TPP-NH2 and the EDCI adduct formed were removed by column chromatography using silica gel. Finally, the pure TPP–PEG–biotin chemical was dried completely using a lyophilizer and characterized by spectroscopy methods. MALDI-TOF MS: m/z 4169.3 [M]+.

TPP-PEG-生物素

TPP-PEG-微生物素的制成借助动物学技术体素-PEG-COOH与TPP胺中间的酸胺偶联反映提炼了TPP-PEG-动物学技术体素药剂学材料。简来讲之,将1 mmol动物学技术体素-PEG-COOH、DMAP、EDCI和3 ml CH2Cl2添加窗户塑料瓶中,反映结合物在0°C下拌和3分钟。进而添加1mmol TPP-NH2,在空调温度放到N2欢乐气氛下连续式拌和反映结合物36分钟。借助热熔胶柱色谱法彻底清除未偶联的TPP-NH2和变成的EDCI加合物。然后,选用冻干机将纯TPP-PEG-动物学技术体素药剂学材料截然非常干燥,并借助光谱图法去分析方法。MALDI-TOF MS:m/z 4169.3[m]+。关联推介:NHS-PEG-COOHACA-PEG-COOHFA-PEG-COOHLA-PEG-COOHIA-PEG-COOHAC-PEG-COOHAlKyne-PEG-COOHCOOH-PEG-EPOCOOH-PEG-MACOOH-PEG-N3CHO-PEG-COOHBiotin-PEG-COOHFmoc-NH-PEG-COOHBoc-NH-PEG-COOHMal-PEG-COOH上述软文主要内容源头各大刊物或文献综述,假如商标侵权请联络你们删出!