TPP-PEG-biotin的合成
论文参考文献:Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†诗人:Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song文章微信链接: 前言: The TPP–PEG–biotin chemical was synthesized by the acid–amine coupling reaction between biotin–PEG–COOH and TPP-amine. Briefly, 1 mmol of biotin–PEG–COOH, DMAP, EDCI and 3 ml of CH2Cl2 were added to a glass vial and the reaction mixture stirred for 3 h at 0 °C. Then 1 mmol of TPP-NH2 was added and the reaction mixture was stirred continuously for 36 h at room temperature under a N2 atmosphere. The unconjugated TPP-NH2 and the EDCI adduct formed were removed by column chromatography using silica gel. Finally, the pure TPP–PEG–biotin chemical was dried completely using a lyophilizer and characterized by spectroscopy methods. MALDI-TOF MS: m/z 4169.3 [M]+.