论文参考文献：Biotin-conjugated PEGylated porphyrin self-assembled nanoparticles co-targeting mitochondria and lysosomes for advanced chemo-photodynamic combination therapy†做者：Baskaran Purushothaman , Jinhyeok Choi , Solji Park , Jeongmin Lee , Annie Agnes Suganya Samson , Sera Hong and Joon Myong Song文章联接：  In this study, biotin-conjugated PEGylated photosensitizer (PS) self-assembled nanoparticles (meso-tetraphenylporphyrin (TPP)–PEG–biotin SANs) were prepared via a self-assembly process to serve as nanocarriers for chemo-drugs as well as PSs. Electron microscopy results reveal the spherical shape of the nanoparticles (NPs). In the NPs, conjugated biotin plays a key role in selective tumor targeting. In vitro cellular experiments revealed the rapid cellular uptake of the TPP–PEG–biotin conjugates by MCF-7 cells that overexpress the biotin receptor, and verified that the conjugates were much more effective PSs than TPPS used as control in the cytotoxicity test. Interestingly, subcellular localization studies showed that the conjugates and their self-assembled NPs were localized mainly in mitochondria and partially in lysosomes, whereas TPPS was localized only in lysosomes. With the exclusive localization in mitochondria, high-content cell based assay showed that the TPP–PEG–biotin SANs induced rapid mitochondrial membrane potential transition (MPT), leading to cellular apoptosis. The chemo-drug doxorubicin (DOX) was successfully encapsulated in the TPP–PEG–biotin SANs (DOX@TPP–PEG–biotin) and had synergistic effects with enhanced cytotoxicity after PDT action. 

在这一项实验中，按照自装设的时候催化合成了动物学素偶联的聚乙二醇化光敏剂（PS）自装设奈米塑料再生颗粒（meso-四苯基卟啉（TPP）-PEG-动物学素-SAN），当做催化药剂和PS的奈米质粒载体。电商体视显微镜没想到显现了纳米级a粒子（NP）的球状。在NP中，共轭怪物素在决定性肉瘤靶向疗法中起着重要的作用。体内神经元实验所具体分析了过于表述怪物素蛋白激酶的MCF-7神经元对TPP-PEG-怪物素偶联物的更快神经元摄食，并验正了偶联物差用作神经元毒副作用经过多次实验发现对比的TPPS更有效性。亚癌人体受损细胞准确wifi精准产品位置定位研发表示，偶联物基本自折装NP一般准确wifi精准产品位置定位上线播放粒体中，部位准确wifi精准产品位置定位在溶酶体中，而TPPS仅准确wifi精准产品位置定位在溶酶体。高的含量癌人体受损细胞监测表示，TPP-PEG-微生物素SANs仅上线播放粒体中准确wifi精准产品位置定位，可引发线粒体膜电极电位快换算（MPT），形成癌人体受损细胞凋亡。化疗药中成药阿霉素（DOX）获得成功打包封装在TPP-PEG-生物体工程素SAN中(DOX@TPP-PEG-生物体工程素），并在PDT用后具提高癌细胞毒素的协用途。相关的推送：ICG-PEG-AlkyneICG-PEG-BiotinICG-PEG-CHOICG-PEG-COOHICG-PEG-cRGDICG-PEG-FolateICG-PEG-MALICG-PEG-N3ICG-PEG-NH2ICG-PEG-NHSICG-PEG-OHICG-PEG-SH