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Bi(mPEG-S-S)-TPE胶束的制备与表征
发布时间:2025-07-14     作者:zyl   分享到:
学术论文:AIE-Featured Redox-Sensitive Micelles for Bioimaging and Efficient Anticancer Drug Delivery小说家:by Wei Zhao 1,†,Zixue Li 1,†,Na Liang 2,*,Jiyang Liu 1,Pengfei Yan 1 andShaoping Sun 专著联接:The drug-loaded Bi(mPEG-S-S)-TPE micelles were obtained as follows. At first, the Bi(mPEG-S-S)-TPE dispersion in distilled water was prepared. Then, the PTX in the acetone solution was added. After being sonicated for 3 min (on for 3 s and off for 2 s), the mixture was dialyzed (MWCO of 3500 Da) against distilled water for 2 h and further filtrated with a 0.45 μm filter to obtain the drug-loaded micelles. The micelles could be further freeze-dried and stored as a white powder.XRD analysis was used to analyze the crystalline characteristic of PTX in the micelles (Geigerflex, Rigaku Co., Tokyo, Japan). Data were collected from 5° to 50° with a step-scan mode. The dynamic light-scattering method was employed to determine the particle size and zeta potential of PTX-loaded micelles (Zetasizer Nano-ZS90, Malvern Instruments, Malvern, UK). The drug loading (DL) and encapsulation efficiency (EE) were calculated using Equations (2) and (3), respectively.

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Bi(mPEG-S-S)-TPE胶束的准备与分析方法载药的Bi(mPEG-S-S)-TPE胶束以下几点提升。前提是,制取了Bi(mPEG-S-S)-TPE在蒸溜水里的乳状液体。接着,加入到二甲苯饱和溶液中的PTX。由5分钟的多普勒彩超进行处理(来3秒,开启2秒)后,将交织物用蒸溜水透析(MWCO为3500 Da)2一小时,接着用0.45μm滤水器进一歩滤水,达到载药胶束。胶束能否进一歩冰冻干澡,即为白色的咖啡豆的行驶存储。XRD分折广泛用于分折胶束中PTX的成果基本特性。选用阶跃扫码模式切换从5°到50°处理数据库。选用gif动态光散射法测定法PTX电动机扭矩电阻胶束的粒度分布和ζ电势差(Zetasizer Nano-ZS90,Malvern Instruments,荷兰Malvern)。不同运用方程式式(2)和(3)确定性药物电动机扭矩电阻量(DL)和包封成功率(EE)。相关内容个性化推荐:Silane-PEG-BiotinLA-PEG-BiotinIA-PEG-BiotinBiotin-PEG-ACAN3-PEG-BiotinOPSS-PEG-BiotinBiotin-PEG-MalBiotin-PEG-SCMSH-PEG-BiotinBiotin-PEG-OHBiotin-PEG-NH2往上原创文章项目源头各种学术期刊或论文参考文献,烦请侵犯知识产权请联络我误删!