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利用16:0 Biotinyl Cap PE构建可结合金纳米粒子的功能化囊泡
发布时间:2025-07-10     作者:kx   分享到:
论文:采用光镊雕琢和融为一体仿生技术囊泡网站外链://www.nature.com/articles/s41467-018-04282-w创作者:吉多·博洛涅西马克·S·弗里丁啊里·萨利希-雷哈尼内森·E·巴洛,尼古拉斯·J·布鲁克斯,奥斯卡·塞斯尤瓦尔·埃拉尼节选:囊泡深度融合和囊泡微化学反应釜要制作功能键化的金nm微粒囊泡,我们大家都先要在包含2 wt.% 16:0 生物技术素帽聚丁二烯 (Biotinyl Cap PE) 的0.5 M白砂糖饱和溶剂中电铸POPC囊泡。当的存在荧光脂质时,其分量为1 wt.%。在钙黄绿素稀释溶解和脂肪酸质表达方式实验所中,囊泡顺利顺利通过精华液相变更(见本文)出现,分量为0.5 M白砂糖/草莓糖高密度梯度方向。下面来,将150 nm链霉亲和素包被的金nm微粒(Nanopartz,荷兰科罗拉多州;厂品C11-150-TS-50;2.5 mg ml −1)以1:9的此例调用到囊泡中,将原辅料涡旋式30一分钟以力促构建。VIM的出现工艺与前同等,但静态饱和溶剂中的NaCl分量为0.25 M。顺利顺利通过在VIM画质上准确把握并产生150 mW(在阱处)或极高耗油率的脉冲激光束来建立囊泡的相融。要逃避突然相融,便用高于100 mW的脉冲激光束(在阱处)对囊泡参与房间调节(多补证明 13)。我们大家都还便用了Aurora-DSGnm微粒(AuNP记号的脂质;1-2 nm;Avanti),当其以2 wt.%的分量植入膜中时,不想發生相融。

16:0 Biotinyl Cap PE

Vesicle fusion and vesicle microreactorsTo prepare functionalised AuNP vesicles, we first electroformed POPC vesicles in 0.5 M sucrose solution with 2 wt.% 16:0 Biotinyl Cap PE. When fluorescent lipids were present, these were at 1 wt.%. In the calcein dilution and protein expression experiments, vesicles were formed via emulsion phase transfer (see above), with 0.5 M sucrose/glucose density gradient. Next, 150 nm streptavidin-coated AuNPs (Nanopartz, CO, USA; product C11-150-TS-50; 2.5 mg ml−1) were added to the vesicles 1:9, with the sample vortexed for 30 min to drive conjugation. VIMs were formed as before, but with 0.25 M NaCl in the external solution. Vesicles were fused by focusing and applying a laser of 150 mW (at trap) or greater on the VIM interface. Vesicles were manipulated in space using a <100 mW laser (at trap) to avoid unintentional fusion (Supplementary Note 13). We also used Aurora-DSG Nanoparticles (AuNP-labelled lipids; 1–2 nm; Avanti), which did not result in fusion when embedded in the membrane at 2 wt.%.杭州pg电子娱乐游戏app 微生物可以提供相应物料:DSPE-PEG-LysozymeStearic acid-PEG-COOHDSPE-PEG-PLGADSPE-PEG-rhuMAb HER2-Fab’or scFv(二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-*靶向疗法蛋清)cRGD-PEG-N3DSPE-PEG-Trastuzumab(二硬脂酰基磷脂酰甲醇胺-聚乙二醇-*靶向疗法蛋清)DSPE-PEG13-TFPDSPE-PEG-LSLERFLRCWSDAPA(二硬脂酰基磷脂酰乙酸乙酯胺-聚乙二醇-*靶向药物血清)DSPE-PEG-CSSRTMHHC(二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶向药物蛋白质)R6H4-PEG-DSPE这稿件项目来历分类杂志或参考文献,如遇知识产权侵权请取得联系各位卸载!