文章：施用光镊圆雕和融为一体防生囊泡网超链接：//www.nature.com/articles/s41467-018-04282-w小说作家：吉多·博洛涅西马克·S·弗里丁蚂蚁金服·萨利希-雷哈尼内森·E·巴洛，尼古拉斯·J·布鲁克斯，奥斯卡·塞斯尤瓦尔·埃拉尼节选：囊泡相融和囊泡微化学物反应器要让制取功能键化的金nm水塑料颗粒囊泡，让我们大家第一在包含2 wt.% 16:0 生物技术素帽聚丁二烯 (Biotinyl Cap PE) 的0.5 M绵白糖稀硫酸中电铸POPC囊泡。当长期存在荧光脂质时，其含水量为1 wt.%。在钙黄绿素做好稀释工作和膳食纤维质表达出来检测中，囊泡能够面霜相转到（见文中）变成，含锌量为0.5 M绵白糖/草莓糖溶解度均值。接到地面上来，将150 nm链霉亲和素包被的金nm水塑料颗粒（Nanopartz，荷兰科罗拉多州；护肤品C11-150-TS-50；2.5 mg ml −1）以1:9的比列加入到囊泡中，将土样涡旋式30半小时以可以淡化紧密联系。VIM的变成步骤与前不同，但外稀硫酸中的NaCl含水量为0.25 M。能够在VIM接面上凝焦并增加150 mW（在阱处）或高些输出的皮秒机光来实现了囊泡的结合。要让预防事故结合，选用低于100 mW的皮秒机光（在阱处）对囊泡来进行面积控制（提供详细说明 13）。让我们大家还选用了Aurora-DSGnm水塑料颗粒（AuNP标出的脂质；1-2 nm；Avanti），当其以2 wt.%的含锌量嵌到膜中时，不是造成结合。

Vesicle fusion and vesicle microreactorsTo prepare functionalised AuNP vesicles, we first electroformed POPC vesicles in 0.5 M sucrose solution with 2 wt.% 16:0 Biotinyl Cap PE. When fluorescent lipids were present, these were at 1 wt.%. In the calcein dilution and protein expression experiments, vesicles were formed via emulsion phase transfer (see above), with 0.5 M sucrose/glucose density gradient. Next, 150 nm streptavidin-coated AuNPs (Nanopartz, CO, USA; product C11-150-TS-50; 2.5 mg ml−1) were added to the vesicles 1:9, with the sample vortexed for 30 min to drive conjugation. VIMs were formed as before, but with 0.25 M NaCl in the external solution. Vesicles were fused by focusing and applying a laser of 150 mW (at trap) or greater on the VIM interface. Vesicles were manipulated in space using a <100 mW laser (at trap) to avoid unintentional fusion (Supplementary Note 13). We also used Aurora-DSG Nanoparticles (AuNP-labelled lipids; 1–2 nm; Avanti), which did not result in fusion when embedded in the membrane at 2 wt.%.成都pg电子娱乐游戏app 生物制品供应关联产品的：DSPE-PEG-LysozymeStearic acid-PEG-COOHDSPE-PEG-PLGADSPE-PEG-rhuMAb HER2-Fab’or scFv（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶向药物蛋白酶)cRGD-PEG-N3DSPE-PEG-Trastuzumab（二硬脂酰基磷脂酰酒精胺-聚乙二醇-*靶向药物核蛋白)DSPE-PEG13-TFPDSPE-PEG-LSLERFLRCWSDAPA（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶向药物淀粉酶)DSPE-PEG-CSSRTMHHC（二硬脂酰基磷脂酰甲醇胺-聚乙二醇-*靶点蛋清)R6H4-PEG-DSPE往上好文章方式來源几大类杂志期刊或资料，比如商标侵权请连续我门移除！