mPEG-DSPE/阿霉素胶束的制备及其淋巴结转移治疗效果研究
资料：MPEG-DSPE水滑石物胶束适用于淋疤结变动的经淋疤放疗联接：//www.sciencedirect.com/science/article/abs/pii/S0378517315002902做者：雪莉，董 青，严志强，维岳 路，冯小编，曹 协 阿，谢作旭，苏冰霞，刘 敏阿节选：本研究探讨备制了甲基聚乙二醇-二硬脂酰磷脂酰工业乙醇胺/阿霉素（MPEG-DSPE/DOX）胶束应用在*淋疤结转回。备制的MPEG-DSPE/DOX胶束呈球型，颗粒直径为20 ± 5nm。A375上皮组织化对DOX和MPEG-DSPE/DOX胶束的摄食率分开 为51.2%和88.7%。RAW264.7上皮组织化对MPEG-DSPE/罗丹明B胶束的毁灭率比专门安全使用罗丹明B低17.2倍。肉里注入液体后，MPEG-DSPE胶束经淋疤管吸纳，在腘窝淋疤结中众多。 MPEG-DSPE/DOX胶束较专门DOX能特别得到缓解注入液体区域肉里组织化的损坏。组建A375上皮组织化淋疤结转回裸鼠整治，肉里注入液体后，MPEG-DSPE/DOX胶束组腘窝淋疤结和髂窝淋疤结权重均特别超过顺利淡盐水组和DOX组。MPEG-DSPE/DOX胶束能有郊攻击腘窝淋疤结和髂窝淋疤结中的*上皮组织化。与此同时提出的，MPEG-DSPE胶束有的是种很有非常好的*淋疤结转回的性药物递送控制系统。 AbstractLymph node metastasis is one of the major pathways for tumor formation and it is difficult to deliver chemotherapeutics at therapeutic concentrations to lymph node metastasis. This study prepared methyl poly(ethylene glycol)-distearoylphosphatidylethanolamine/doxorubicin (MPEG-DSPE/DOX) micelle for the treatment of lymph node metastasis. The MPEG-DSPE/DOX micelle prepared were of spherical morphology with a particle size of 20 ± 5 nm. The uptake rates of DOX and MPEG-DSPE/DOX micelle by A375 cells were 51.2% and 88.7%, respectively. The phagocytosis rate of MPEG-DSPE/Rhodamine B micelle by RAW264.7 cells was 17.2-fold lower than for Rhodamine B alone. After subcutaneous injection, MPEG-DSPE micelle underwent lymphatic absorption and accumulated in popliteal lymph nodes. MPEG-DSPE/DOX micelle significantly alleviated damage to the subcutaneous tissue of the injection sites compared with DOX alone. We established a model of nude mice bearing lymph node metastasis of A375 cells. After subcutaneous injection, the weights of both the popliteal and iliac lymph nodes of the MPEG-DSPE/DOX micelle group were significantly lower than in the saline and DOX groups. MPEG-DSPE/DOX micelle effectively killed the tumor cells in popliteal and iliac lymph nodes. In conclusion, MPEG-DSPE micelle is a promising drug delivery system for the treatment of lymph node metastasis.
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