DOPE-PEG-Mal的合成与纯化方法
文献综述：一同抑制 CD47 和 PD-L1 可增强学习后性情性和认知性恶性肿瘤免疫性作用以其受损细胞指数公式移除联结：//www.thelancet.com/article/S2352-3964(19)30158-6/fulltext小说作者：舒 莲，谢锐 志，叶玉英，谢晓东，李淑慧，路玉生，李碧飞，程云龙，弗拉基米尔·卡塔纳耶夫，李嘉节选：MAL-PEG-DOPE的分解成MAL-PEG-DOPE的提炼选取过去发布的内容。主要包括EDC/NHS科技将MAL-PEG-COOH的羧基与DOPE的胺基通过。实际技巧如下所示：将30mg羧基淡化的PEG溶水二氯二氧化氮中，并与5mgEDC和4mgNHS混合式，空调温度下反复绞拌2h。第二参与8mg DOPE（MAL-PEG-COOH∶DOPE=1∶1，摩尔比），离氮气保护的下生理响应包夜。将生理响应乙酰乙酸在高速回转汽化仪中潮湿至大那部分二氯二氧化氮，第二参与冷乙腈中。未生理响应的DOPE在2414g下离心力10min，不溶水冷乙腈。上清液在高速回转汽化仪中潮湿为稀脂质。将膜用DD水好多化。将生理响应乙酰乙酸倒入透析袋（大分子量= 8 k Da）中，并转变至50 mL DD水悬浊液中，空调温度下生理响应48个小时，区分解离的EDC/NHS/MAL-PEG-COOH。以后乙酰乙酸DOPE-PEG-MAL接着随后用冻干机冰冻。要确认DOPE-PEG-MAL的通过，对检样开展了核磁振动波谱探讨。Synthesis of MAL-PEG-DOPESynthesis of MAL-PEG-DOPE refers to previously published articles [22,23]. The conjugation of carboxyl groups of MAL-PEG-COOH to the amine groups of DOPE was accomplished using the EDC/NHS technique. The process was carried out as follows: 30 mg carboxyl-modified PEG was dissolved in dichloromethane and mixed with EDC (5 mg) and NHS (4 mg). The solution was stirred continuously for 2 h at room temperature. Subsequently, 8 mg DOPE (MAL-PEG-COOH: DOPE =1:1, molar ratio) was added, and the reaction proceeded overnight under nitrogen. The reaction product was dried out most dichloromethane in rotary evaporator and then added to cold acetonitrile. The unreacted DOPE was centrifuged at 2414g for 10 min which was insoluble in cold acetonitrile. And the supernatant was dried to thin lipid in rotary evaporator. The film was hydrated with DD water. The reaction product was enclosed in dialysis bag (MW = 8 k Da) and transferred into 50 mL of DD water solution to separate free EDC/ NHS/ MAL-PEG-COOH at room temperature for 48 h. The final product DOPE-PEG-MAL was subsequently freezed by lyophilizer. To confirm the DOPE-PEG-MAL conjugation, the samples were examined by nuclear magnetic resonance spectroscopy.
pg电子娱乐游戏app
微信公众号
官方微信