文献资料：Surface Modification of Erythrocytes with Lipid Anchors: Structure–Activity Relationship for Optimal Membrane Incorporation, in vivo Retention, and Immunocompatibility原作者：Hanmant Gaikwad, Guankui Wang, Yue Li, David Bourne, Dmitri Simberg资料链接代码： Thus, RBCs labeled with diacyl glycerol derivative Cy3-C12 and phospholipid Cy3-DSPE showed much faster removal than stable DiI-C12. To compare the stability of different lipids in vitro, we measured the fluorescence of labeled RBCs after incubation in mouse serum for 3 h. According to Figure  5A, DiI-C18, DiI-C18:2, DiI-PEG3400Mtz, DiI-C12, Cy3-C12, and Cy3-cholesterol RBCs showed less than 15% loss in MFI at 3 h. At the same time, Cy3-DSPE RBCs showed over 60% loss of MFI. Confocal microscopy showed that Cy3-DSPE and DiI-C18 had similar uniform labeling of RBCs prior to incubation in serum (Figure 5B). After 3 h incubation in serum, there was five times more fluorescence released in serum from Cy3-DSPE RBCs than from DiI-C18 RBCs (Figure 5C). Thin layer chromatography (TLC) analysis showed the presence of intact Cy3-DSPE along with some degradation products (Figure 5D). While 3 h time incubation is shorter than the in vivo longevity of some of the lipids (Figure 3B), the data indicate the removal of the phospholipid from RBC membrane through interaction with serum.

用二酰基甘油发展物Cy3-C12和磷脂Cy3-DSPE标上的红神经受损癌组织体现出比维持的DiI-C12变快的剔除转速。很各种不同脂质在 在身体外，我们大家测试了标上的红神经受损癌组织在小鼠血清中孵育3小时候后的荧光 h.DiI-C18、DiI-C18:2、DiI-PEG3400Mtz、DiI-C12、Cy3-C12和Cy3固醇红神经受损癌组织在3℃时MFI损耗不大于15% h.与此同时，Cy3-DSPE红神经受损癌组织MFI损耗高出60%。共自动对焦电子显微镜显视，Cy3-DSPE和DiI-C18在血清中孵育前对红神经元拥有相类似的均箭头（图5B）。3在此之后 在血清中孵育h后，Cy3-DSPE红神经元在血清中发挥的荧单是DiI-C18红神经元的5倍。薄层色谱（TLC）阐述显现发生完成的Cy3-DSPE和有些溶解化合物（图5D）。然而3 h孵鸡耗时短于in 有些脂质的休内期限，数据统计阐明磷脂实现与血清的相护帮助从红血细胞去除。相应的推建：Cy3-DPPECy3-DSPECy3-PEG-DOPECy3-PEG-DPPECy5-DOPECy5-DPPECy5-DSPECy5-PEG-DOPECy5-PEG-DPPE以上方式方式方式主要来源各个杂志或资料，以免知识产权侵权请连接我们的移除！