期刊论文：还具有资料粘液渗透到性的巴氏奶外泌体能用的 于口服药递送siRNA友链：//pubs.rsc.org/en/content/articlehtml/2020/bm/d0bm01497d作著：Matthew R. Warren a、 Chenzhen 张 a、 Armin Vedadghavami a、 Krister Bokvist b、 Pradeep K. Dhal c和 Ambika G. Bajpayee * ad节选：荧光共轭 DSPE-PEG-叠氮化物的配制。 食用DSPE-PEG-叠氮化物（一些简称为DPA）在 mExo 的从表面养成疏水放 PEG 表层。首选将 DPA 以 2 mg mL −1的稀硫酸浓硫酸质量氨水浓度可溶性高，溶于水的在 DMSO 中，接着逐滴加入到 1× PBS 中并一直的搅拌器器，使 DPA 的之后稀硫酸浓硫酸质量氨水浓度为 0.02 mg mL −1（7 μM），但是配制水性树脂聚氨酯贮备稀硫酸。这对触及 DPA 稀硫酸浓硫酸质量氨水浓度程序化的探索（即校正 mExo 的从表面额定负载和锚安全稳相关性探索），DPA经由叠氮化物-DBCO 点击率进入率化学上的发应与荧光地方 DBCO-Cy5 联系。将 DBCO-Cy5（DMSO 中稀硫酸浓硫酸质量氨水浓度为 1 mg mL −1）逐滴加入到水性树脂聚氨酯 DPA 贮备液中，一直的搅拌器器，使之后摩尔之比 1 [细分隔符（1/6-em）]：[细分隔符（1/6-em）]4。接着在室内温度下猛地摆动该稀硫酸以使得点击率进入率发应点击率进入率发应后，将含带DPA-Cy5和量过大DBCO-Cy5的稀硫酸简单加入到外泌体打样定制中使用放。古诗网：Preparation of fluorescent-conjugated DSPE-PEG-Azide. DSPE-PEG-Azide (hereby DPA) was utilized to enable a hydrophobically-inserted PEG coating on the mExo surface. An aqueous stock solution was prepared by first dissolving DPA in DMSO at 2 mg mL−1, followed by dropwise addition to 1× PBS with continuous stirring for a final DPA concentration of 0.02 mg mL−1 (7 μM). For studies involving quantification of DPA concentration (i.e. measurement of mExo surface loading and anchor stability studies), DPA was conjugated to the fluorescent moiety DBCO-Cy5 via the azide-DBCO click chemistry reaction. DBCO-Cy5 (1 mg mL−1 in DMSO) was added dropwise with continuous stirring to aqueous DPA stock for a 1[thin space (1/6-em)]:[thin space (1/6-em)]4 final molar ratio. This solution was then incubated for 6 h at room temperature with light shaking to facilitate the click reaction. After the click reaction, the solution containing DPA-Cy5 and excess DBCO-Cy5 was added directly to exosome samples for insertion.

北京pg电子娱乐游戏app 微生物展示想关新产品：DSPE-PEG-Cy5DSPE-PEG-mAb 2C5（二硬脂酰基磷脂酰工业乙醇胺-聚乙二醇-*靶向治疗蛋白酶)DSPE-PEG-OPSSCy5-DSPEDSPE-PEG-TetrazineDSPE-PEG-DAAsp6-PEG-DSPEDSPE-PEG2000-NGRXpeptide-PEG-DSPE以上的原创文章内部从何而来各论文期刊或期刊论文，深表歉意侵犯著作权请搞好关系当我们删除图片！