论文：Ultrafast Diffusion of a Fluorescent Cholesterol Analog in Compartmentalized Plasma Membranes写作者：Nao Hiramoto-Yamaki, Kenji A. K. Tanaka, Kenichi G. N. Suzuki, Koichiro M. Hirosawa, Manami S. H. Miyahara, Ziya Kalay, Koichiro Tanaka, Rinshi S. Kasai … See all authors 专著外部链接： Cholesterol distribution and dynamics in the plasma membrane (PM) are poorly understood. The recent development of Bodipy488-conjugated cholesterol molecule (Bdp-Chol) allowed us to study cholesterol behavior in the PM, using single fluorescent-molecule imaging. Surprisingly, in the intact PM, Bdp-Chol diffused at the fastest rate ever found for any molecules in the PM, with a median diffusion coefficient (D) of 3.4 µm2/second, which was ∼10 times greater than that of non-raft phospholipid molecules (0.33 µm2/second), despite Bdp-Chol's probable association with raft domains. Furthermore, Bdp-Chol exhibited no sign of entrapment in time scales longer than 0.5 milliseconds. In the blebbed PM, where actin filaments were largely depleted, Bdp-Chol and Cy3-conjugated dioleoylphosphatidylethanolamine (Cy3-DOPE) diffused at comparable Ds (medians = 5.8 and 6.2 µm2/second, respectively), indicating that the actin-based membrane skeleton reduces the D of Bdp-Chol only by a factor of ∼2 from that in the blebbed PM, whereas it reduces the D of Cy3-DOPE by a factor of ∼20. These results are consistent with the previously proposed model, in which the PM is compartmentalized by the actin-based membrane-skeleton fence and its associated transmembrane picket proteins for the macroscopic diffusion of all of the membrane molecules, and suggest that the probability of Bdp-Chol passing through the compartment boundaries, once it enters the boundary, is ∼10× greater than that of Cy3-DOPE. Since the compartment sizes are greater than those of the putative raft domains, we conclude that raft domains coexist with membrane-skeleton-induced compartments and are contained within them.

Bodipy488共轭低密度低密度胆固醇分子式结构（Bdp-Chol）的提升使我们都还可以实用单荧光分子式结构影像的研究PM中的低密度低密度胆固醇的行为。中值散出常数（D）为3.4µm2/秒，比非筏磷脂团伙（0.33µm2/十几秒）大10倍，也许Bdp-Chul会与筏节构域业内。还有，Bdp-Chol在不超0.5的时间间隔标准上没能表現出被困与此，无法离开的征象 毫秒。在泡状PM中，肌动蛋清酶丝大量耗完，Bdp-Chol和Cy3综合的二油酰基磷脂酰工业乙醇胺（Cy3-DOPE）以等同于的Ds（中十位数）扩散作用 = 5.8 和6.2µm2/秒），揭示应用于肌动蛋清酶的膜骨架仅将Bdp-Chol的D大大减少了泡状PM的约2倍，而将Cy3-DOPE的D大大减少约20倍。这部分成果与之间提出了的模特同步，在该模特中，PM被来源于肌动淀粉酶的膜骨架栅栏门还有有关于的跨膜尖桩淀粉酶分格，应用在大部分膜原子核的外部经济外扩散，并呈现Bdp-Chol万一打开界线，凭借隔室界线的概率统计比Cy3-DOPE有可能10倍。基于隔室尺寸规格高于假定的筏域，企业看出依据，筏域与膜骨架引导的隔室相融，并是指在这之中。

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