期刊论文：Simultaneous blocking of CD47 and PD-L1 increases innate and adaptive cancer immune responses and cytokine release做者：Shu Liana ∙ Ruizhi Xiea ∙ Yuying Yeb ∙ Xiaodong Xiea ∙ Shuhui Lia ∙ Yusheng Lua,c ∙ et al. Show more参考文献链接代码: 引言：Synthesis of MAL-PEG-DOPE refers to previously published articles. The conjugation of carboxyl groups of MAL-PEG-COOH to the amine groups of DOPE was accomplished using the EDC/NHS technique. The process was carried out as follows: 30 mg carboxyl-modified PEG was dissolved in dichloromethane and mixed with EDC (5 mg) and NHS (4 mg). The solution was stirred continuously for 2 h at room temperature. Subsequently, 8 mg DOPE (MAL-PEG-COOH: DOPE =1:1, molar ratio) was added, and the reaction proceeded overnight under nitrogen. The reaction product was dried out most dichloromethane in rotary evaporator and then added to cold acetonitrile. The unreacted DOPE was centrifuged at 2414g for 10 min which was insoluble in cold acetonitrile. And the supernatant was dried to thin lipid in rotary evaporator. The film was hydrated with DD water. The reaction product was enclosed in dialysis bag (MW = 8 k Da) and transferred into 50 mL of DD water solution to separate free EDC/ NHS/ MAL-PEG-COOH at room temperature for 48 h. The final product DOPE-PEG-MAL was subsequently freezed by lyophilizer. To confirm the DOPE-PEG-MAL conjugation, the samples were examined by nuclear magnetic resonance spectroscopy.

MAL-PEG-DOPE的制成MAL-PEG-DOPE的合并规范了开始之前刊发的经典文章。的使用EDC/NHS系统来了MAL-PEG-COOH羧基与DOPE胺基的偶联。该全过程按下述手段来：30 将mg羧基掩盖的PEG融化在二氯二氧化氮中，并与EDC（5mg）和NHS（4mg）比调。将稀硫酸不间断搅拌器2 h在温度下。那么，8 入驻mg DOPE（MAL-PEG-COH:DOPE=1:1，摩尔比），在氢气下体现留宿。体现货物在平移蒸发掉器中干澡出大位置二氯甲烷气体，第二入驻冷乙腈中。将未作用的DOPE在2414g下离心区分10分鐘 min，其不不溶冷乙腈。上清液在平移多效冷却器中潮湿成薄脂质。用DD水使保护膜水合。将作用货物装进透析袋（MW = 8 k Da）并存入50 mL DD水盐溶液，在室内温度下区分徘徊的EDC/NNHS/MAL-PEG-COOH 48每小时 h.紧接着用冻干机速冻最后货物DOPE-PEG-MAL。为了能证明DOPE-PEG-MAL共轭，用核磁振动光谱分析对土样做出了判断。关于介绍：DOPE-PEG-MalDOPE-PEG-NH2DOPE-PEG-SCDOPE-PEIDPPE-Cap-BiotinDPPE-Cap-FolateDPPE-ICGDPPE-mPEGDPPE-PEG2-NH-Mal之上原创文章文章种类以及期刊论文或文章，若有侵权商标请连系我门册除！