文献：DOPC-Detergent Conjugates: Fusogenic Carriers for Improved In Vitro and In Vivo Gene Delivery

笔者：Philippe Pierrat, Anne Casset, Dimitri Kereselidze, Marie Lux, Françoise Pons, Luc Lebeau论文参考文献图片链接： 论文摘要：Phospholipid-detergent conjugates are proposed as fusogenic carriers for gene delivery. Eleven compounds are prepared and their properties are investigated. The ability of the conjugates to promote fusion with a negatively charged model membrane is determined. Their DNA delivery efficiency and cytotoxicity are assessed in vitro. Lipoplexes are administered in the mouse lung, and transgene expression Indeterminate inflammatory activity are measured. The results show that conjugation of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) with C12E4 produces a carrier that can efficiently deliver DNA to cells, with negligible ­associated toxicity. Fusogenicity of the conjugates shows good correlation with in vitro transfection efficiency and crucially depends on the length of the polyether moiety of the detergent. Finally, DOPC-C12E4 reveals highly potent for in vivo DNA delivery and favorably compares to GL67A, the current golden standard for gene delivery to the airway, opening the way for further promising developments.

磷脂洗衣剂偶联物被提出者做基因遗传递送的融入形式。制取了11种类化合物，并对其经营性质来了研究方案。测得偶联物带动与带负电势的仿真模型膜融入的水平。在身体外分析评估其的DNA递送速率和神经细胞渗透性。在小鼠肺用肥Lipoplex，并測量转什么是基因表答不确实的细菌感染化学活化。后果意味着，1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）与C12E4的偶联产生一个多种质粒载体，可不行有用地将DNA递寄到内部，相关联致毒可不行失去不记。偶联物的融和原性与体内转染速率存在好的想关性，还有在过大阶段上考量于洗滌剂聚醚那部分的粗度。最好，DOPC-C12E4在体中DNA递送个方面体现经验丰富，与迄今为止在呼吸道dna递送的金标准GL67A相较极具优势，为进一次有发展方向的发展方向开发了之路。相应选择：DOPE-PEG-CH2COOHDOPE-PEG-FADOPE-PEG-MalDOPE-PEG-NH2DOPE-PEG-SCDOPE-PEIDPPE-Cap-BiotinDPPE-Cap-FolateDPPE-ICGDPPE-mPEGDPPE-PEG2-NH-Mal以上方面一篇文章方面原因各种论文期刊或论文资料，如果图片侵权请链接各位刪除！