DSPE-PEG2000-MAL在多种纳米杂化物构建中的应用
专著：Dual Functioned Hexapeptide-Coated Lipid-Core Nanomicelles Suppress Toll-Like Receptor-Mediated Inflammatory Responses through Endotoxin Scavenging and Endosomal pH Modulation我 ：Yuting Ji, Liya Sun, Yuan Liu, Yanhui Li, Tongxuan Li, Jiameng Gong, Xiali Liu, Huiqiang Ma, Jingying Wang, Bing Chen, Shan-Yu Fung, Hong Yang医学文献下载链接：引言：
To overcome this problem, we constructed three versions of peptide (Pep12)-modified nano-hybrids by replacing the GNP core with different cores that were made of clinically applicable materials in this study (Figure 1a): M-P12, Lipo-P12, and PLGA-P12. We anticipated that these new cores may also bring additional functionality to the nano-hybrids. M-P12 was made of self-assembled distearoyl-phosphatidylethanolamine-poly(ethylene glycol) (2000)-maleimide (DSPE-PEG2000-MAL) nanomicelles (M-MAL) that were conjugated with Pep12 (CLPFFD) on the surface by Michael addition reaction between the maleimide of the DSPE-PEG2000-MAL and the thiol group of the cysteine (C) residue at the N-terminal of Pep12 (Figure 1b). The nuclear magnetic resonance hydrogen spectroscopy (1H NMR) was applied to confirm the conjugation of Pep12 on the nanomicelle surface. The disappearance of the maleimide peak (at 6.7 ppm) in DSPE-PEG2000-MAL and the appearance of the benzene peak of the peptide (at 7.1–7.2 ppm) in DSPE-PEG2000-Pep12 suggested the complete conjugation of Pep12 to the nanomicelle (Figure S1a, Supporting Information). Lipo-P12 was fabricated by inserting DSPE-PEG2000-Pep12 into the phospholipid bilayer of the DSPE liposomes (Lipo). PLGA-P12 was constructed by conjugating Pep12 to PLGA monomers via amide bond formation (Figure 1b), followed by nano-precipitation to form peptide-modified PLGA nanoparticles. The appearance of the benzene peak in the NMR spectra of PLGA-P12 suggested the successful conjugation of Pep12 to PLGA (Figure S2a, Supporting Information).
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