论文资料：应用原子结构力谱法考评束搏1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱膜的杨氏模量联结：//pubs.acs.org/doi/abs/10.1021/jp505451h创作者：王曦，罗伯特·N·桑德森‡雷吉娜·拉根论文摘要：将由氧溶液溶液浓度在0 mol %至24 mol %两者的1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱 (POPC) 和的不同氧溶液溶液浓度的1,2-二硬脂酰-sn-甘油-3-磷酸工业乙醇胺-N-聚乙二醇-2000- N- [3-(2-吡啶基二硫代)丙酸酯] (DSPE-PEG-PDP) 組成的单双层线路囊泡装配在共价键级平整度好的文档模板脱离金 (TS Au) 表面上。动用共价键力显微镜观察 (AFM) 对抗震液下列得绳束脂质双层线路膜 (tLBM) 做好力谱定量分析，带来了对於机械装备积极响应随绳束大分子 DSPE-PEG-PDP 氧溶液溶液浓度转变的数据信息。杨氏模量完成操作近期修改图片的 Sneddon 类别的身材曲线拟合力-毛边的身材曲线来肯定，该类别矫正了产自左上方基本材料的功劳。在低氧化还原电位下，杨氏模量降到可以的 POPC LBM（即可以直接坐落于胶体肌底上）。模量的限制归因于膜纯净水性的新增，可能完成掺进 DSPE-PEG-PDP 约束基团，tLBM 和胶体肌底区间内的交叉耦合限制了。从测定方法的杨氏模量值可以知道，氧化还原电位要高于 6 mol % 时，PEG 链构象打算 tLBM 的KBK刚性。AFM 力谱的数据剖析得出结论，聚乙二醇 (PEG) 鸡油菌到毛刷的变为再次发生在 6 mol % 附过，这使得 tLBM 在挺高的 DSPE-PEG-PDP 氧化还原电位下先变的软，接着时不时变硬，这与变为有观。当 DSPE-PEG-PDP 氧化还原电位新增到 24 mol % 时，AFM 形貌和杨氏模量仿佛与另外一个个相变着法子关； AFM 形貌图形与多层盘型式同一，中间 DSPE-PEG-PDP 隔离霜在盘的顶部。

AbstractUnilamellar vesicles composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) with varying 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethylene glycol)-2000-N-[3-(2-pyridyldithio) propionate] (DSPE-PEG-PDP) concentration between 0 mol % and 24 mol % were assembled on atomically flat template-stripped gold (TS Au) surfaces. Force spectroscopy, using an atomic force microscope (AFM), of the resulting tethered lipid bilayer membranes (tLBMs) in buffer provided information regarding mechanical response as a function of tethering molecule, DSPE-PEG-PDP, concentration. Young’s modulus was determined by fitting the force–indentation curve with a recently modified Sneddon model that corrects for contributions from the substrate underneath. At low concentrations, Young’s modulus is lower than that of a supported POPC LBM, i.e., directly sitting on a solid substrate. The decrease in modulus is attributed to increased membrane fluidity as coupling between the tLBM and solid substrate is reduced by the incorporation of DSPE-PEG-PDP tethering groups. From the determined Young’s modulus values, the PEG chain conformation is found to dominate tLBM rigidity at concentrations above 6 mol %. Analysis of AFM force spectroscopy data indicates that the poly(ethylene glycol) (PEG) mushroom to brush transition occurs near 6 mol %, and this leads to first softening and then abrupt stiffening of tLBMs at higher DSPE-PEG-PDP concentration associated with the transition. When DSPE-PEG-PDP concentration is increased to 24 mol %, AFM topography and Young’s modulus appear correlated with another phase transition; AFM topography images are consistent with a bilayer disk structure with DSPE-PEG-PDP segregated at the rim of the disk.深圳pg电子娱乐游戏app 生物技术带来了各种相关设备：DSPE-PEG-Cy5.5DSPE-PEG-Cy7DSPE-PEG-NBDDSPE-PEG-TRITCDSPE-PEG-ComarinDSPE-PEG-ICGDSPE-PEG-PyreneDSPE-PEG-Ce6DSPE-PEG-Cy3上面好的文章主要内容特征几大类中文核心期刊或医学文献，比如抄袭请关联自己删除图片！