DSPE-PEG-PDP辅助制备的柔性PEG垫层用于脂质双层膜重构研究
文章:聚乙二醇电动机扭矩脂质单层在金从表面的原位形成了及分析方法图片链接://pubs.acs.org/doi/abs/10.1021/la048378o写作者:杰弗里·C·芒罗,柯蒂斯·W·弗兰克绪论:会有人提出者,在脂质双重线路膜和固体颗粒面区间内修改配位聚苯胺垫层,也可以行成绵软、可变性形的膜层,导致实现了跨膜蛋白酶的添加图片和迁出。本设计在第二步树脂离心分离操作过程,在聚乙二醇 (PEG) 承载上行成了可迁出的、可束搏的脂质双重线路膜。 PEG 薄膜和珍珠棉是在共树脂离心分离异基本系统远爪 PEG 脂质配位聚苯胺(1,2-二硬脂酰-sn-甘油-3-磷酸甲醇胺-N-聚(乙二醇)-2000- N- [3-(2-(吡啶基二硫代)丙酸酯])(DSPE-PEG-PDP)和非脂质基本系统化的 PEG-PDP 从甲醇/水饱和溶液物中分离纯化的,如仍然的开题报告(Munro,JC;Frank,CW Langmuir 2004,20,3339-3349)下列述。但是便用三步脂质吸收方式。先,将脂质从己烷水溶液中吸收到 PEG 承载上。二、,将囊泡吸收并溶合在单单从外层以在水生态中构成单层。光洗白实验设计后的荧光完全恢复展示,该的过程生产传播因子约为 2 μm /s。根据束搏脂质密度计算公式的提高,单层膜的渗透率略为降。单单从外层等亚铁离子体振动法(用做核查原位膜的厚度)和荧光法(用做酶联免疫法核查每家18 x 18毫米(mm)打样定制英文的荧光比强度)也印证了单层膜的构成,而不是两层架构。悲伤的是,荧光显微镜体检也展示打样定制英文上会有更大的偏差,这规定了该系统化的应用性。Abstract ImageInclusion of a polymer cushion between a lipid bilayer membrane and a solid surface has been suggested as a means to provide a soft, deformable layer that will allow for transmembrane protein insertion and mobility. In this study, mobile, tethered lipid bilayers were formed on a poly(ethylene glycol) (PEG) support via a two-step adsorption process. The PEG films were prepared by coadsorbing a heterofunctional, telechelic PEG lipopolymer (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-poly(ethylene glycol)-2000-N-[3-(2-(pyridyldithio)propionate]) (DSPE-PEG-PDP) and a nonlipid functionalized PEG-PDP from an ethanol/water mixture, as described in a previous paper (Munro, J. C.; Frank, C. W. Langmuir2004, 20, 3339−3349). Then a two-step lipid adsorption strategy was used. First, lipids were adsorbed onto the PEG support from a hexane solution. Second, vesicles were adsorbed and fused on the surface to create a bilayer in an aqueous environment. Fluorescence recovery after photobleaching experiments show that this process results in mobile bilayers with diffusion coefficients on the order of 2 μm2/s. The mobility of the bilayers is decreased slightly by increasing the density of tethered lipids. The formation of bilayers, and not multilayer structures, is also confirmed by surface plasmon resonance, which was used to determine in situ film thickness, and by fluorimetry, which was used to determine quantitatively the fluorescence intensity for each 18 by 18 mm sample. Unfortunately, fluorescence microscopy also shows that there are large defects on the samples, which limits the utility of this system.



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