文献：Design of pH-sensitive methotrexate prodrug-targeted curcumin nanoparticles for efficient dual-drug delivery and combination cancer therapy

论文参考文献友链：//www.tandfonline.com/doi/full/10.2147/IJN.S152312#d1e205小编：Jiajiang Xie,Zhongxiong Fan,Yang Li,Yinying Zhang,Fei Yu,Guanghao Su

结语：

To investigate the intracellular drug/nanocarrier delivery of MTX-Imine-M-CUR, Cy5.5-labeled DSPE-PEG was synthesized for use as a near-infrared fluorescent probe (DSPE-PEG-Cy5.5 was conjugated between DSPE-PEG-NH2 and Cy5.5 NHS ester via amide linkage). HeLa cells were seeded into 24-well plates at a density of 1.0×104 cells per well. After culturing for 24 h, the cells were treated with MTX-Imine-MCy5.5-CUR at 37°C for predetermined incubation time periods. Afterward, the cells were washed three times with PBS and fixed with 4% (v/v) paraformaldehyde for 15 min. Finally, the cells were washed by cold PBS twice, stained with DAPI for 10 min, and observed using a Leica TCS SP5 CLSM.

因为科学研究MTX-Imine-M-CUR的细胞核内药/奈米媒介递送，自动合成了Cy5.5标识的DSPE-PEG充当近红外荧光检测器（DSPE-PEG-Cy5.5凭借酰胺键通过在DSPE-PEG-NH2和Cy5.5 NHS酯区间内）。HeLa血生殖组织组织细胞以每孔1.0×104个血生殖组织组织细胞的溶解度育苗到24孔板中。激发24天后，在37°C使用MTX-Imine-MCy5.5-CUR加工血生殖组织组织细胞订座的孵育时间间隔。其次，用PBS洗涤剂血生殖组织组织细胞三遍，用4%（v/v）多聚室内甲醛进行固定1430分钟。末尾，用冷PBS清洗上皮细胞几次，用DAPI染色法10几分钟，并选择Leica TCS SP5 CLSM关注。有关的介绍好产品：Cy7-PEG-DPPEDSPE-PEG-Cy7DSPE-PEG-cRGDDPPE-PEG-cRGDDMPE-PEG-cRGDDOPE-PEG-cRGDDPPE-PEG-RGDDMPE-PEG-RGDDOPE-PEG-RGD4-Arm PEG-DSPEC12-PEG-COOH以下主要内容主要内容渠道各项中文核心期刊或论文参考文献，予以抄袭请取得关于我们的删了！