DSPE-PEG-COOH用于制备DSPE-PEG-DOX的相关文献（含合成路线）

期刊论文名: Peptide Engraftment on PEGylated Nanoliposomes for Bone Specific Delivery of PTH (1-34) in Osteoporosis小说作家：Taewon Hwang 1, Hee Dong Han, Chung Kil Song, Hasoo Seong, Jung Hyun Kim, Xiaoyuan Chen, Byung Cheol Shin内容提要：防癌肿癌中成药的肿癌特喜欢的人递送是肿癌中成药规划设计的时候中要面对的首要挑战中的一种。在一项实验中，我能够将新获得的DSPE-PEG2000-DOX（DPD）当做脂质物质添加脂质体中备制了阿霉素（DOX）偶联脂质体（DCL），并检测了其自身抗肿癌渗透性。能够酰胺键将DOX偶联到DSPE-PEG2000-COOH上获得了DPD，并能够（1）H-NMR阐述认可了获得的DPD的电化学结构类型。能够聚酯薄膜浇铸水非法备制了脂质体规格尺寸为130nm的DCL。流式的神经元术阐述否认，DCL的神经元摄食很大超过老式脂质体。DCL对小鼠B16F10自然黑着色剂瘤荷瘤小鼠的抗肿癌渗透性显示，DCL比老式脂质体更很好地缓和肿癌生张，这或许是根据DOX介导的内吞的时候。 

实验使用方法:

Synthesis ofConjugate (SDSSD-DSPE)A lipid-PEG-peptide combination that may subsequently be added to liposomes forbone targeting was developed, SDSSD was conjugated to DSPE-PEG2000-C00H usingcarbodiimide chemistry, based on the previously described method by Wang et al. withmodi fications [32. Briefly, DSPE-PEG2000-COOH was dissolved in 0.1 M MES bufferat pH 6.0 and stirred at 2-8 °C for 10 min. Then, EDC.HCl and NHS (20 equivalentseach) were added to the reaction mixture and stirred for 60 min at 2-8 °C. A 7.4 pHadjustment was made to the reaction mixture, Finally, SDSSD, previously dissolved inMilli-Q water, was added to the reaction mixture and stirred overnight. The resultantmixture was dialyzed using a 2KD dialysis membrane for 48 h to remove unconjugatedSDSSD. Purifield SDSSD-DSPE (conjugate) was freeze-dried and characterized by'H-NMRMaldi-TOF, and a Scanning Electron Microscope (SEM) followed by Energy Dispersive。

 

  

反应结构路线

 

重要检测:

文献核磁

 

主要实验设计没想到代表Synthesis of DSPE-PEG2000-DOX (DPD)Covalent conjugation of DOX to DSPE.PEG2000-COOH was confirmed by HNMR (500 MHz, Auto Sampler-HRMAS.FT-NMR, Bruker, Switzerland) analysis ofDPD. 'HNMR spectra were obtained forcomb polymer solutions in deuteratedchloroform. As shown in a Figure 3, theproton peaks of DOX (1,2 and 3)andDSPE-PEG2000-C00H(4 and 5)wereobserved at 7.4~8.2 ppm and 1.2~3.7ppm, respectively.

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生产商:昆明pg电子娱乐游戏app 生物体信息技术较少子公司

应用:科研课题

情况：液体/粉化/氢氧化钠溶液

产址:石家庄

溫馨消息提醒:仅作科技创新，不应用于体内科学试验!

有关于货品：

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